7eyo
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==Crystal structure of leech hyaluronidase== | ==Crystal structure of leech hyaluronidase== | ||
| - | <StructureSection load='7eyo' size='340' side='right'caption='[[7eyo]]' scene=''> | + | <StructureSection load='7eyo' size='340' side='right'caption='[[7eyo]], [[Resolution|resolution]] 1.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYO FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYO FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyo OCA], [https://pdbe.org/7eyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyo RCSB], [https://www.ebi.ac.uk/pdbsum/7eyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyo ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyo OCA], [https://pdbe.org/7eyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyo RCSB], [https://www.ebi.ac.uk/pdbsum/7eyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hyaluronidases have attracted a great deal of interest in the field of medicine due to their fundamental roles in the breakdown of hyaluronan. However, little is known about the catalytic mechanism of the hyaluronate 3-glycanohydrolases. Here, we report the crystal structure and cleavage pattern of a leech hyaluronidase (LHyal), which hydrolyzes the beta-1,3-glycosidic bonds of hyaluronan. LHyal exhibits the typical structural features of glycoside hydrolase 79 family but contains a variable 'exo-pocket' loop where basic residues R102 and K103 are the structural determinants of hyaluronan binding. Through analysis of the hydrolysis of even- and odd-numbered hyaluronan oligosaccharides, we demonstrate that hexasaccharide is the shortest natural substrate, which can be cleaved from both the reducing and non-reducing ends to release disaccharides, and pentasaccharides are the smallest fragments for recognition and hydrolysis. These observations provide new insights into the degradation of hyaluronan and the evolutionary relationships of the GH79 family enzymes. | ||
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| + | Structure and cleavage pattern of a hyaluronate 3-glycanohydrolase in the glycoside hydrolase 79 family.,Huang H, Hou X, Xu R, Deng Z, Wang Y, Du G, Rao Y, Chen J, Kang Z Carbohydr Polym. 2022 Feb 1;277:118838. doi: 10.1016/j.carbpol.2021.118838. Epub , 2021 Nov 2. PMID:34893255<ref>PMID:34893255</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7eyo" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Hyaluronidase 3D structures|Hyaluronidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of leech hyaluronidase
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Categories: Large Structures | Hou XD | Huang H | Kang Z | Rao YJ
