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7qfz

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Current revision (07:53, 1 May 2024) (edit) (undo)
 
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<StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
<StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7qfz]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7qfz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_fergusonii_ATCC_35469 Escherichia fergusonii ATCC 35469]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B7L3Y3_ESCF3 B7L3Y3_ESCF3]
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Bacteria are under constant assault by bacteriophages and other mobile genetic elements. As a result, bacteria have evolved a multitude of systems that protect from attack. Genes encoding bacterial defence mechanisms can be clustered into 'defence islands', providing a potentially synergistic level of protection against a wider range of assailants. However, there is a comparative paucity of information on how expression of these defence systems is controlled. Here, we functionally characterize a transcriptional regulator, BrxR, encoded within a recently described phage defence island from a multidrug resistant plasmid of the emerging pathogen Escherichia fergusonii. Using a combination of reporters and electrophoretic mobility shift assays, we discovered that BrxR acts as a repressor. We present the structure of BrxR to 2.15 A, the first structure of this family of transcription factors, and pinpoint a likely binding site for ligands within the WYL-domain. Bioinformatic analyses demonstrated that BrxR-family homologues are widespread amongst bacteria. About half (48%) of identified BrxR homologues were co-localized with a diverse array of known phage defence systems, either alone or clustered into defence islands. BrxR is a novel regulator that reveals a common mechanism for controlling the expression of the bacterial phage defence arsenal.
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A widespread family of WYL-domain transcriptional regulators co-localizes with diverse phage defence systems and islands.,Picton DM, Harling-Lee JD, Duffner SJ, Went SC, Morgan RD, Hinton JCD, Blower TR Nucleic Acids Res. 2022 May 20;50(9):5191-5207. doi: 10.1093/nar/gkac334. PMID:35544231<ref>PMID:35544231</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7qfz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Escherichia fergusonii ATCC 35469]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Blower, T R]]
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[[Category: Blower TR]]
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[[Category: Picton, D M]]
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[[Category: Picton DM]]
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[[Category: Dna binding]]
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[[Category: Dna binding protein]]
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[[Category: Phage defence]]
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[[Category: Transcription factor]]
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Current revision

BrxR, a WYL-domain containing transcriptional regulator

PDB ID 7qfz

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