8d4a
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Cas12a2 quaternary complex== | |
| + | <StructureSection load='8d4a' size='340' side='right'caption='[[8d4a]], [[Resolution|resolution]] 2.74Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8d4a]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfuricurvum_sp._PC08-66 Sulfuricurvum sp. PC08-66] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D4A FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.74Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d4a OCA], [https://pdbe.org/8d4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d4a RCSB], [https://www.ebi.ac.uk/pdbsum/8d4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d4a ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0C2W1L1_9BACT A0A0C2W1L1_9BACT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded DNA following recognition of a complementary RNA target, culminating in abortive infection(1). Here we report structures of Cas12a2 in binary, ternary and quaternary complexes to reveal a complete activation pathway. Our structures reveal that Cas12a2 is autoinhibited until binding a cognate RNA target, which exposes the RuvC active site within a large, positively charged cleft. Double-stranded DNA substrates are captured through duplex distortion and local melting, stabilized by pairs of 'aromatic clamp' residues that are crucial for double-stranded DNA degradation and in vivo immune system function. Our work provides a structural basis for this mechanism of abortive infection to achieve population-level immunity, which can be leveraged to create rational mutants that degrade a spectrum of collateral substrates. | ||
| - | + | RNA targeting unleashes indiscriminate nuclease activity of CRISPR-Cas12a2.,Bravo JPK, Hallmark T, Naegle B, Beisel CL, Jackson RN, Taylor DW Nature. 2023 Jan;613(7944):582-587. doi: 10.1038/s41586-022-05560-w. Epub 2023 , Jan 4. PMID:36599980<ref>PMID:36599980</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8d4a" style="background-color:#fffaf0;"></div> |
| - | [[Category: Taylor | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sulfuricurvum sp. PC08-66]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Bravo JPK]] | ||
| + | [[Category: Taylor DW]] | ||
Current revision
Cas12a2 quaternary complex
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