7pgi
From Proteopedia
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==NaVAb1p (bicelles)== | ==NaVAb1p (bicelles)== | ||
| - | <StructureSection load='7pgi' size='340' side='right'caption='[[7pgi]]' scene=''> | + | <StructureSection load='7pgi' size='340' side='right'caption='[[7pgi]], [[Resolution|resolution]] 3.64Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7pgi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcanivorax_borkumensis_SK2 Alcanivorax borkumensis SK2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGI FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgi OCA], [https://pdbe.org/7pgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgi RCSB], [https://www.ebi.ac.uk/pdbsum/7pgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgi ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.638Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgi OCA], [https://pdbe.org/7pgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgi RCSB], [https://www.ebi.ac.uk/pdbsum/7pgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q0VNY2_ALCBS Q0VNY2_ALCBS] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Every voltage-gated ion channel (VGIC) has a pore domain (PD) made from four subunits, each comprising an antiparallel transmembrane helix pair bridged by a loop. The extent to which PD subunit structure requires quaternary interactions is unclear. Here, we present crystal structures of a set of bacterial voltage-gated sodium channel (BacNaV) 'pore only' proteins that reveal a surprising collection of non-canonical quaternary arrangements in which the PD tertiary structure is maintained. This context-independent structural robustness, supported by molecular dynamics simulations, indicates that VGIC-PD tertiary structure is independent of quaternary interactions. This fold occurs throughout the VGIC superfamily and in diverse transmembrane and soluble proteins. Strikingly, characterization of PD subunit-binding Fabs indicates that non-canonical quaternary PD conformations can occur in full-length VGICs. Together, our data demonstrate that the VGIC-PD is an autonomously folded unit. This property has implications for VGIC biogenesis, understanding functional states, de novo channel design, and VGIC structural origins. | ||
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| + | Quaternary structure independent folding of voltage-gated ion channel pore domain subunits.,Arrigoni C, Lolicato M, Shaya D, Rohaim A, Findeisen F, Fong LK, Colleran CM, Dominik P, Kim SS, Schuermann JP, DeGrado WF, Grabe M, Kossiakoff AA, Minor DL Jr Nat Struct Mol Biol. 2022 Jun 2. pii: 10.1038/s41594-022-00775-x. doi:, 10.1038/s41594-022-00775-x. PMID:35655098<ref>PMID:35655098</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7pgi" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Alcanivorax borkumensis SK2]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Arrigoni C]] | [[Category: Arrigoni C]] | ||
[[Category: Lolicato M]] | [[Category: Lolicato M]] | ||
Current revision
NaVAb1p (bicelles)
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