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| | <StructureSection load='3qg7' size='340' side='right'caption='[[3qg7]], [[Resolution|resolution]] 2.78Å' scene=''> | | <StructureSection load='3qg7' size='340' side='right'caption='[[3qg7]], [[Resolution|resolution]] 2.78Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qg7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QG7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qg7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QG7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.78Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qg6|3qg6]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qg7 OCA], [https://pdbe.org/3qg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qg7 RCSB], [https://www.ebi.ac.uk/pdbsum/3qg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qg7 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qg7 OCA], [https://pdbe.org/3qg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qg7 RCSB], [https://www.ebi.ac.uk/pdbsum/3qg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qg7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Janda, J D]] | + | [[Category: Janda JD]] |
| - | [[Category: Kaufmann, G F]] | + | [[Category: Kaufmann GF]] |
| - | [[Category: Kirchdoerfer, R K]] | + | [[Category: Kirchdoerfer RK]] |
| - | [[Category: Wilson, I A]] | + | [[Category: Wilson IA]] |
| - | [[Category: Aip4]]
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| - | [[Category: Antigen recognition]]
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| - | [[Category: Immune system]]
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| - | [[Category: Immunoglobulin fold]]
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| - | [[Category: Secreted]]
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| Structural highlights
Publication Abstract from PubMed
In the postantibiotic era, available treatment options for severe bacterial infections caused by methicillin-resistant Staphylococcus aureus have become limited. Therefore, new and innovative approaches are needed to combat such life-threatening infections. Virulence factor expression in S. aureus is regulated in a cell density-dependent manner using "quorum sensing," which involves generation and secretion of autoinducing peptides (AIPs) into the surrounding environment to activate a bacterial sensor kinase at a particular threshold concentration. Mouse monoclonal antibody AP4-24H11 was shown previously to blunt quorum sensing-mediated changes in gene expression in vitro and protect mice from a lethal dose of S. aureus by sequestering the AIP signal. We have elucidated the crystal structure of the AP4-24H11 Fab in complex with AIP-4 at 2.5 A resolution to determine its mechanism of ligand recognition. A key Glu(H95) provides much of the binding specificity through formation of hydrogen bonds with each of the four amide nitrogens in the AIP-4 macrocyclic ring. Importantly, these structural data give clues as to the interactions between the cognate staphylococcal AIP receptors AgrC and the AIPs, as AP4-24H11.AIP-4 binding recapitulates features that have been proposed for AgrC-AIP recognition. Additionally, these structural insights may enable the engineering of AIP cross-reactive antibodies or quorum quenching vaccines for use in active or passive immunotherapy for prevention or treatment of S. aureus infections.
Structural Basis for Ligand Recognition and Discrimination of a Quorum-quenching Antibody.,Kirchdoerfer RN, Garner AL, Flack CE, Mee JM, Horswill AR, Janda KD, Kaufmann GF, Wilson IA J Biol Chem. 2011 May 13;286(19):17351-8. Epub 2011 Mar 23. PMID:21454495[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kirchdoerfer RN, Garner AL, Flack CE, Mee JM, Horswill AR, Janda KD, Kaufmann GF, Wilson IA. Structural Basis for Ligand Recognition and Discrimination of a Quorum-quenching Antibody. J Biol Chem. 2011 May 13;286(19):17351-8. Epub 2011 Mar 23. PMID:21454495 doi:10.1074/jbc.M111.231258
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