7q4q
From Proteopedia
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| - | ==== | + | ==Magacizumab Fab fragment in complex with human LRG1 epitope== |
| - | <StructureSection load='7q4q' size='340' side='right'caption='[[7q4q]]' scene=''> | + | <StructureSection load='7q4q' size='340' side='right'caption='[[7q4q]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7q4q]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q4Q FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q4q OCA], [https://pdbe.org/7q4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q4q RCSB], [https://www.ebi.ac.uk/pdbsum/7q4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q4q ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q4q OCA], [https://pdbe.org/7q4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q4q RCSB], [https://www.ebi.ac.uk/pdbsum/7q4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q4q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A2GL_HUMAN A2GL_HUMAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The formation of new dysfunctional blood vessels is a crucial stage in the development of various conditions such as macular degeneration, diabetes, cardiovascular disease, neurological disease and inflammatory disorders, as well as during tumor growth, eventually contributing to metastasis. An important factor involved in pathogenic angiogenesis is leucine-rich alpha-2-glycoprotein 1 (LRG1), the antibody blockade of which has been shown to lead to a reduction in both choroidal neovascularization and tumor growth in mouse models. In this work, the structural interactions between the LRG1 epitope and the Fab fragment of Magacizumab, a humanized function-blocking IgG4 against LRG1, are analysed, determining its specific binding mode and the key residues involved in LRG1 recognition. Based on these structural findings, a series of mutations are suggested that could be introduced into Magacizumab to increase its affinity for LRG1, as well as a model of the entire Fab-LRG1 complex that could enlighten new strategies to enhance affinity, consequently leading towards an even more efficient therapeutic. | ||
| + | |||
| + | Structural basis of human LRG1 recognition by Magacizumab, a humanized monoclonal antibody with therapeutic potential.,Gutierrez-Fernandez J, Javaid F, De Rossi G, Chudasama V, Greenwood J, Moss SE, Luecke H Acta Crystallogr D Struct Biol. 2022 Jun 1;78(Pt 6):725-734. doi: , 10.1107/S2059798322004132. Epub 2022 May 9. PMID:35647920<ref>PMID:35647920</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7q4q" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| + | [[Category: Gutierrez-Fernandez J]] | ||
| + | [[Category: Luecke H]] | ||
Current revision
Magacizumab Fab fragment in complex with human LRG1 epitope
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