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7q1g

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<StructureSection load='7q1g' size='340' side='right'caption='[[7q1g]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='7q1g' size='340' side='right'caption='[[7q1g]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7q1g]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6sng 6sng]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q1G FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7q1g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae_B728a Pseudomonas syringae pv. syringae B728a]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4bgo 4bgo] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6sng 6sng]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q1G FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q1g OCA], [https://pdbe.org/7q1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q1g RCSB], [https://www.ebi.ac.uk/pdbsum/7q1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q1g ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q1g OCA], [https://pdbe.org/7q1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q1g RCSB], [https://www.ebi.ac.uk/pdbsum/7q1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q1g ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q4ZR20_PSEU2 Q4ZR20_PSEU2]
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EfeUOB/M has been characterised in Pseudomonas syringae pathovar. syringae as a novel type of ferrous-iron transporter, consisting of an inner-membrane protein (EfeUPsy) and three periplasmic proteins (EfeOPsy, EfeMPsy and EfeBPsy). The role of an iron permease and peroxidase function has been identified for the EfeU and EfeB proteins, respectively, but the role of EfeO/M remains unclear. EfeMPsy is an 'M75-only' EfeO-like protein with a C-terminal peptidase-M75 domain (EfeOII/EfeM family). Herein, we report the 1.6 A resolution crystal structure of EfeMPsy, the first structural report for an EfeM component of P. syringae pv. syringae. The structure possesses the bi-lobate architecture found in other bacterial periplasmic substrate/solute binding proteins. Metal binding studies, using SRCD and ICP-OES, reveal a preference of EfeMPsy for copper, iron and zinc. This work provides detailed knowledge of the structural scaffold, the metal site geometry, and the divalent metal binding potential of EfeM. This work provides crucial underpinning for a more detailed understanding of the role of EfeM/EfeO proteins and the peptidase-M75 domains in EfeUOB/M iron uptake systems in bacteria.
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Crystal structure and metal binding properties of the periplasmic iron component EfeM from Pseudomonas syringae EfeUOB/M iron-transport system.,Rajasekaran MB, Hussain R, Siligardi G, Andrews SC, Watson KA Biometals. 2022 Mar 29. pii: 10.1007/s10534-022-00389-2. doi:, 10.1007/s10534-022-00389-2. PMID:35348940<ref>PMID:35348940</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7q1g" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Rajasekaran, M]]
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[[Category: Pseudomonas syringae pv. syringae B728a]]
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[[Category: Watson, K A]]
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[[Category: Rajasekaran M]]
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[[Category: Efem]]
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[[Category: Watson KA]]
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[[Category: Efeuob iron-transport system]]
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[[Category: Metal transport]]
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[[Category: Metal-binding]]
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[[Category: Peptidase-m75 domain]]
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Current revision

Crystal structure and metal binding properties of the periplasmic iron component EfeM from Pseudomonas syringae EfeUOB/M iron-transport system

PDB ID 7q1g

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