3re1

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Current revision (08:35, 20 March 2024) (edit) (undo)
 
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<StructureSection load='3re1' size='340' side='right'caption='[[3re1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3re1' size='340' side='right'caption='[[3re1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3re1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Psesm Psesm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RE1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3re1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._tomato_str._DC3000 Pseudomonas syringae pv. tomato str. DC3000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RE1 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hemD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223283 PSESM])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Uroporphyrinogen-III_synthase Uroporphyrinogen-III synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.75 4.2.1.75] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3re1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3re1 OCA], [https://pdbe.org/3re1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3re1 RCSB], [https://www.ebi.ac.uk/pdbsum/3re1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3re1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3re1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3re1 OCA], [https://pdbe.org/3re1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3re1 RCSB], [https://www.ebi.ac.uk/pdbsum/3re1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3re1 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q88B90_PSESM Q88B90_PSESM]
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Uroporphyrinogen III synthase (U3S) is one of the key enzymes in the biosynthesis of tetrapyrrole compounds. It catalyzes the cyclization of the linear hydroxymethylbilane (HMB) to uroporphyrinogen III (uro'gen III). We have determined the crystal structure of U3S from Pseudomonas syringae pv. tomato DC3000 (psU3S) at 2.5A resolution by the single wavelength anomalous dispersion (SAD) method. Each psU3S molecule consists of two domains interlinked by a two-stranded antiparallel beta-sheet. The conformation of psU3S is different from its homologous proteins because of the flexibility of the linker between the two domains, which might be related to this enzyme's catalytic properties. Based on mutation and activity analysis, a key residue, Arg219, was found to be important for the catalytic activity of psU3S. Mutation of Arg219 to Ala caused a decrease in enzymatic activity to about 25% that of the wild type enzyme. Our results provide the structural basis and biochemical evidence to further elucidate the catalytic mechanism of U3S.
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Crystal structure of uroporphyrinogen III synthase from Pseudomonas syringae pv. tomato DC3000.,Peng S, Zhang H, Gao Y, Pan X, Cao P, Li M, Chang W Biochem Biophys Res Commun. 2011 May 20;408(4):576-81. Epub 2011 Apr 19. PMID:21527255<ref>PMID:21527255</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3re1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Psesm]]
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[[Category: Pseudomonas syringae pv. tomato str. DC3000]]
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[[Category: Uroporphyrinogen-III synthase]]
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[[Category: Chang WR]]
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[[Category: Chang, W R]]
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[[Category: Li M]]
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[[Category: Li, M]]
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[[Category: Peng SX]]
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[[Category: Peng, S X]]
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[[Category: Hemd-like family]]
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[[Category: Hmb]]
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[[Category: Lyase]]
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[[Category: Uroporphyrinogen iii synthase]]
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Current revision

Crystal structure of uroporphyrinogen III synthase from Pseudomonas syringae pv. tomato DC3000

PDB ID 3re1

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