3rfe

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of glycoprotein GPIb ectodomain

Structural highlights

3rfe is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.245Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

GP1BB_HUMAN Bernard-Soulier syndrome;22q11.2 deletion syndrome;Fetal and neonatal alloimmune thrombocytopenia. The disease is caused by mutations affecting the gene represented in this entry.

Function

GP1BB_HUMAN Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium.

Publication Abstract from PubMed

Platelet GPIb-IX receptor complex has 3 subunits GPIbalpha, GPIbbeta, and GPIX, which assemble with a ratio of 1:2:1. Dysfunction in surface expression of the complex leads to Bernard-Soulier syndrome. We have crystallized the GPIbbeta ectodomain (GPIbbeta(E)) and determined the structure to show a single leucine-rich repeat with N- and C-terminal disulphide-bonded capping regions. The structure of a chimera of GPIbbeta(E) and 3 loops (a,b,c) taken from the GPIX ectodomain sequence was also determined. The chimera (GPIbbeta(Eabc)), but not GPIbbeta(E), forms a tetramer in the crystal, showing a quaternary interface between GPIbbeta and GPIX. Central to this interface is residue Tyr106 from GPIbbeta, which inserts into a pocket generated by 2 loops (b,c) from GPIX. Mutagenesis studies confirmed this interface as a valid representation of interactions between GPIbbeta and GPIX in the full-length complex. Eight GPIbbeta missense mutations identified from patients with Bernard-Soulier syndrome were examined for changes to GPIb-IX complex surface expression. Two mutations, A108P and P74R, were found to maintain normal secretion/folding of GPIbbeta(E) but were unable to support GPIX surface expression. The close structural proximity of these mutations to Tyr106 and the GPIbbeta(E) interface with GPIX indicates they disrupt the quaternary organization of the GPIb-IX complex.

Quaternary organization of GPIb-IX complex and insights into Bernard-Soulier syndrome revealed by the structures of GPIbbeta and a GPIbbeta/GPIX chimera.,McEwan PA, Yang W, Carr KH, Mo X, Zheng X, Li R, Emsley J Blood. 2011 Nov 10;118(19):5292-301. Epub 2011 Sep 8. PMID:21908432^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ McEwan PA, Yang W, Carr KH, Mo X, Zheng X, Li R, Emsley J. Quaternary organization of GPIb-IX complex and insights into Bernard-Soulier syndrome revealed by the structures of GPIbbeta and a GPIbbeta/GPIX chimera. Blood. 2011 Nov 10;118(19):5292-301. Epub 2011 Sep 8. PMID:21908432 doi:10.1182/blood-2011-05-356253

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rfe"

@@ Line 3: / Line 3: @@
 <StructureSection load='3rfe' size='340' side='right'caption='[[3rfe]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3rfe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RFE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3rfe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RFE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.245&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rez|3rez]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GP1BB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rfe OCA], [https://pdbe.org/3rfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rfe RCSB], [https://www.ebi.ac.uk/pdbsum/3rfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rfe ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[https://www.uniprot.org/uniprot/GP1BB_HUMAN GP1BB_HUMAN]] Bernard-Soulier syndrome;22q11.2 deletion syndrome;Fetal and neonatal alloimmune thrombocytopenia. The disease is caused by mutations affecting the gene represented in this entry.
+[https://www.uniprot.org/uniprot/GP1BB_HUMAN GP1BB_HUMAN] Bernard-Soulier syndrome;22q11.2 deletion syndrome;Fetal and neonatal alloimmune thrombocytopenia. The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[https://www.uniprot.org/uniprot/GP1BB_HUMAN GP1BB_HUMAN]] Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium.
+[https://www.uniprot.org/uniprot/GP1BB_HUMAN GP1BB_HUMAN] Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 </div>
 <div class="pdbe-citations 3rfe" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Platelet glycoprotein|Platelet glycoprotein]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Carr, K H]]
+[[Category: Carr KH]]
-[[Category: Emsley, J]]
+[[Category: Emsley J]]
-[[Category: Li, R]]
+[[Category: Li R]]
-[[Category: McEwan, P A]]
+[[Category: McEwan PA]]
-[[Category: Mo, X]]
+[[Category: Mo X]]
-[[Category: Yang, W]]
+[[Category: Yang W]]
-[[Category: Zheng, X]]
+[[Category: Zheng X]]
-[[Category: Cell adhesion]]
-[[Category: Glycoprotein]]
-[[Category: Gpix]]
-[[Category: Platelet surface receptor]]

3rfe

From Proteopedia

Current revision

Crystal structure of glycoprotein GPIb ectodomain

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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