Uridylate-specific endoribonuclease
From Proteopedia
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'''Uridylate-specific endoribonuclease (NendoU)''' | '''Uridylate-specific endoribonuclease (NendoU)''' | ||
| - | '''SARS-CoV-2 non-structural protein 15''' (Nsp15) is a nidoviral RNA '''uridylate-specific endoribonuclease''' (NendoU). Its C-terminal catalytic domain belongs to the EndoU family, meaning it produces 2’-3’ cyclic phosphodiester and 5’-hydroxytermini following RNA endonuclease activity on single- and double-stranded RNA and is specific for uridine <ref>PMID:21422822</ref>. Mn2+ dependence has been observed in other members of the NendoU subfamily. | + | '''SARS-CoV-2 non-structural protein 15''' ('''Nsp15''') is a nidoviral RNA '''uridylate-specific endoribonuclease''' (NendoU). Its C-terminal catalytic domain belongs to the EndoU family, meaning it produces 2’-3’ cyclic phosphodiester and 5’-hydroxytermini following RNA endonuclease activity on single- and double-stranded RNA and is specific for uridine <ref>PMID:21422822</ref>. Mn2+ dependence has been observed in other members of the NendoU subfamily. |
The exact functional relevance of Nsp15 is currently unknown. Nsp15-deficient corona viruses remain viable and replicating <ref name="structure">PMID:32304108</ref>. However, conflicting studies have been published on Nsp15’s role in interfering with the innate immune response <ref>PMID:28484023</ref>,<ref>PMID:31351410</ref> and it has been suggested that Nsp15 degrades viral RNA as a method to hide it from host defences <ref name="structure" />. | The exact functional relevance of Nsp15 is currently unknown. Nsp15-deficient corona viruses remain viable and replicating <ref name="structure">PMID:32304108</ref>. However, conflicting studies have been published on Nsp15’s role in interfering with the innate immune response <ref>PMID:28484023</ref>,<ref>PMID:31351410</ref> and it has been suggested that Nsp15 degrades viral RNA as a method to hide it from host defences <ref name="structure" />. | ||
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | [[6vww]], [[6w01]], [[6xdh]], [[7k9p]], [[7keg]], [[7keh]], [[7kf4]] – SCv2NendoU – SARS-CoV-2 <br /> | + | [[5sbf]], [[6vww]], [[6w01]], [[6xdh]], [[7k9p]], [[7keg]], [[7keh]], [[7kf4]] – SCv2NendoU – SARS-CoV-2 <br /> |
[[7me0]], [[7rb0]], [[7rb2]] – SCv2NendoU - Cryo EM<br /> | [[7me0]], [[7rb0]], [[7rb2]] – SCv2NendoU - Cryo EM<br /> | ||
| - | [[2ozk]], [[2rhb]], [[4rs4]], [[4s1t]] – SCv2NendoU (mutant)<br /> | + | [[2ozk]], [[2rhb]], [[4rs4]], [[4s1t]], [[8d34]], [[8u2x]] – SCv2NendoU (mutant)<br /> |
[[6wlc]], [[6x4i]], [[7k0r]], [[7k1l]], [[7k1o]] – SCv2NendoU + uridine derivative<br /> | [[6wlc]], [[6x4i]], [[7k0r]], [[7k1l]], [[7k1o]] – SCv2NendoU + uridine derivative<br /> | ||
| - | [[5s6y]], [[5s72]], [[7n7r]], [[7n7w]] – SCv2NendoU + urea derivative<br /> | + | [[5s6y]], [[5s72]], [[5sai]], [[7n7r]], [[7n7w]] – SCv2NendoU + urea derivative<br /> |
| - | [[5s71]] – | + | [[5s71]] – SCv2NendoU + thiothymidine<br /> |
| - | [[5s6x]], [[5s6z]], [[5s70]] – SCv2NendoU + imidazole derivative<br /> | + | [[5sa5]], [[5sag]], [[5sah]], [[5s6x]], [[5s6z]], [[5s70]] – SCv2NendoU + imidazole derivative<br /> |
| - | [[7n7u]] – SCv2NendoU + pyrimidine derivative<br /> | + | [[5sae]], [[5saf]], [[5sah]], [[7n7u]] – SCv2NendoU + pyrimidine derivative<br /> |
| - | [[7n7y]], [[7n83]] – SCv2NendoU + inhibitor<br /> | + | [[5sa4]], [[5sa6]], [[5sa7]], [[5sa8]], [[5sa9]], [[5saa]], [[5sab]], [[5sac]], [[5sad]], [[7n7y]], [[7n83]] – SCv2NendoU + inhibitor<br /> |
[[6wxc]] – SCv2NendoU + drug<br /> | [[6wxc]] – SCv2NendoU + drug<br /> | ||
[[6x1b]] – SCv2NendoU + DNA<br /> | [[6x1b]] – SCv2NendoU + DNA<br /> | ||
| - | [[7n06]], | + | [[7n06]], [[7n33]], [[7tj2]], [[7tqv]] – SCv2NendoU + RNA – Cryo EM<br /> |
[[5yvd]] – NendoU – MERS coronavirus<br /> | [[5yvd]] – NendoU – MERS coronavirus<br /> | ||
__NOTOC__ | __NOTOC__ | ||
Current revision
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References
- ↑ Ulferts R, Ziebuhr J. Nidovirus ribonucleases: Structures and functions in viral replication. RNA Biol. 2011 Mar-Apr;8(2):295-304. doi: 10.4161/rna.8.2.15196. Epub 2011 Mar 1. PMID:21422822 doi:http://dx.doi.org/10.4161/rna.8.2.15196
- ↑ 2.0 2.1 2.2 2.3 Kim Y, Jedrzejczak R, Maltseva NI, Wilamowski M, Endres M, Godzik A, Michalska K, Joachimiak A. Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Protein Sci. 2020 Apr 17. doi: 10.1002/pro.3873. PMID:32304108 doi:http://dx.doi.org/10.1002/pro.3873
- ↑ Deng X, Hackbart M, Mettelman RC, O'Brien A, Mielech AM, Yi G, Kao CC, Baker SC. Coronavirus nonstructural protein 15 mediates evasion of dsRNA sensors and limits apoptosis in macrophages. Proc Natl Acad Sci U S A. 2017 May 23;114(21):E4251-E4260. doi:, 10.1073/pnas.1618310114. Epub 2017 May 8. PMID:28484023 doi:http://dx.doi.org/10.1073/pnas.1618310114
- ↑ Liu X, Fang P, Fang L, Hong Y, Zhu X, Wang D, Peng G, Xiao S. Porcine deltacoronavirus nsp15 antagonizes interferon-beta production independently of its endoribonuclease activity. Mol Immunol. 2019 Oct;114:100-107. doi: 10.1016/j.molimm.2019.07.003. Epub 2019, Jul 24. PMID:31351410 doi:http://dx.doi.org/10.1016/j.molimm.2019.07.003
