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1fdq

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(New page: 200px<br /> <applet load="1fdq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fdq, resolution 2.1&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1fdq.gif|left|200px]]<br />
 
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<applet load="1fdq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fdq, resolution 2.1&Aring;" />
 
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'''CRYSTAL STRUCTURE OF HUMAN BRAIN FATTY ACID BINDING PROTEIN'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF HUMAN BRAIN FATTY ACID BINDING PROTEIN==
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Expression of brain fatty acid-binding protein (B-FABP) is spatially and, temporally correlated with neuronal differentiation during brain, development. Isothermal titration calorimetry demonstrates that, recombinant human B-FABP clearly exhibits high affinity for the, polyunsaturated n-3 fatty acids alpha-linolenic acid, eicosapentaenoic, acid, docosahexaenoic acid, and for monounsaturated n-9 oleic acid (K(d), from 28 to 53 nm) over polyunsaturated n-6 fatty acids, linoleic acid, and, arachidonic acid (K(d) from 115 to 206 nm). B-FABP has low binding, affinity for saturated long chain fatty acids. The three-dimensional, structure of recombinant human B-FABP in complex with oleic acid shows, that the oleic acid hydrocarbon tail assumes a "U-shaped" conformation, whereas in the complex with docosahexaenoic acid the hydrocarbon tail, adopts a helical conformation. A comparison of the three-dimensional, structures and binding properties of human B-FABP with other homologous, FABPs, indicates that the binding specificity is in part the result of, nonconserved amino acid Phe(104), which interacts with double bonds, present in the lipid hydrocarbon tail. In this context, analysis of the, primary and tertiary structures of human B-FABP provides a rationale for, its high affinity and specificity for polyunsaturated fatty acids. The, expression of B-FABP in glial cells and its high affinity for, docosahexaenoic acid, which is known to be an important component of, neuronal membranes, points toward a role for B-FABP in supplying brain, abundant fatty acids to the developing neuron.
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<StructureSection load='1fdq' size='340' side='right'caption='[[1fdq]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fdq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FDQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HXA:DOCOSA-4,7,10,13,16,19-HEXAENOIC+ACID'>HXA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fdq OCA], [https://pdbe.org/1fdq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fdq RCSB], [https://www.ebi.ac.uk/pdbsum/1fdq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fdq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FABP7_HUMAN FABP7_HUMAN] B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/1fdq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fdq ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: Infundibular hypoplasia and hypopituitarism OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=313430 313430]], Mental retardation, X-linked, with isolated growth hormone deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=313430 313430]]
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*[[Fatty acid-binding protein 3D structures|Fatty acid-binding protein 3D structures]]
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__TOC__
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==About this Structure==
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</StructureSection>
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1FDQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HXA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FDQ OCA].
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==Reference==
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Crystal structure and thermodynamic analysis of human brain fatty acid-binding protein., Balendiran GK, Schnutgen F, Scapin G, Borchers T, Xhong N, Lim K, Godbout R, Spener F, Sacchettini JC, J Biol Chem. 2000 Sep 1;275(35):27045-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10854433 10854433]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Balendiran, G.K.]]
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[[Category: Balendiran GK]]
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[[Category: HXA]]
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[[Category: long chain poly unsaturated fatty acid]]
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[[Category: n-3]]
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[[Category: omega-3]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:51:59 2007''
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Current revision

CRYSTAL STRUCTURE OF HUMAN BRAIN FATTY ACID BINDING PROTEIN

PDB ID 1fdq

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