7xf1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal strucutre of apoCasDinG in complex with ssDNA== | |
| + | <StructureSection load='7xf1' size='340' side='right'caption='[[7xf1]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7xf1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XF1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xf1 OCA], [https://pdbe.org/7xf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xf1 RCSB], [https://www.ebi.ac.uk/pdbsum/7xf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xf1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type IV CRISPR-Cas systems, which are primarily found on plasmids and exhibit a strong plasmid-targeting preference, are the only one of the six known CRISPR-Cas types for which the mechanistic details of their function remain unknown. Here, we provide high-resolution functional snapshots of type IV-A Csf complexes before and after target dsDNA binding, either in the absence or presence of CasDinG, revealing the mechanisms underlying Csf(crRNA) complex assembly, "DWN" PAM-dependent dsDNA targeting, R-loop formation, and CasDinG recruitment. Furthermore, we establish that CasDinG, a signature DinG family helicase, harbors ssDNA-stimulated ATPase activity and ATP-dependent 5'-3' DNA helicase activity. In addition, we show that CasDinG unwinds the non-target strand (NTS) and target strand (TS) of target dsDNA from the Csf(crRNA) complex. These molecular details advance our mechanistic understanding of type IV-A CRISPR-Csf function and should enable Csf complexes to be harnessed as genome-engineering tools for biotechnological applications. | ||
| - | + | Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment.,Cui N, Zhang JT, Liu Y, Liu Y, Liu XY, Wang C, Huang H, Jia N Mol Cell. 2023 Jul 20;83(14):2493-2508.e5. doi: 10.1016/j.molcel.2023.05.036. , Epub 2023 Jun 20. PMID:37343553<ref>PMID:37343553</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7xf1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Cui N]] | ||
| + | [[Category: Huang HD]] | ||
| + | [[Category: Jia N]] | ||
| + | [[Category: Liu YR]] | ||
| + | [[Category: Zhang JT]] | ||
Current revision
Crystal strucutre of apoCasDinG in complex with ssDNA
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