1hi4

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[[Image:1hi4.gif|left|200px]]
 
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==Eosinophil-derived Neurotoxin (EDN) - Adenosien-3'-5'-Diphosphate Complex==
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The line below this paragraph, containing "STRUCTURE_1hi4", creates the "Structure Box" on the page.
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<StructureSection load='1hi4' size='340' side='right'caption='[[1hi4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hi4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HI4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>
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{{STRUCTURE_1hi4| PDB=1hi4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hi4 OCA], [https://pdbe.org/1hi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hi4 RCSB], [https://www.ebi.ac.uk/pdbsum/1hi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hi4 ProSAT]</span></td></tr>
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</table>
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'''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-3'-5'-DIPHOSPHATE COMPLEX'''
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS2_HUMAN RNAS2_HUMAN] This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.<ref>PMID:3458170</ref> <ref>PMID:12578357</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Eosinophil-derived neurotoxin (EDN), a basic ribonuclease found in the large specific granules of eosinophils, belongs to the pancreatic RNase A family. Although its physiological function is still unclear, it has been shown that EDN is a neurotoxin capable of inducing the Gordon phenomenon in rabbits. EDN is also a potent helminthotoxin and can mediate antiviral activity of eosinophils against isolated virions of the respiratory syncytial virus. EDN is a catalytically efficient RNase sharing similar substrate specificity with pancreatic RNase A with its ribonucleolytic activity being absolutely essential for its neurotoxic, helminthotoxic, and antiviral activities. The crystal structure of recombinant human EDN in the unliganded form has been determined previously (Mosimann, S. C., Newton, D. L., Youle, R. J., and James, M. N. G. (1996) J. Mol. Biol. 260, 540-552). We have now determined high resolution (1.8 A) crystal structures for EDN in complex with adenosine-3',5'-diphosphate (3',5'-ADP), adenosine-2',5'-di-phosphate (2',5'-ADP), adenosine-5'-diphosphate (5'-ADP) as well as for a native structure in the presence of sulfate refined at 1.6 A. The inhibition constant of these mononucleotides for EDN has been determined. The structures present the first detailed picture of differences between EDN and RNase A in substrate recognition at the ribonucleolytic active site. They also provide a starting point for the design of tight-binding inhibitors, which may be used to restrain the RNase activity of EDN.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/1hi4_consurf.spt"</scriptWhenChecked>
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1HI4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI4 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors., Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR, J Biol Chem. 2001 May 4;276(18):15009-17. Epub 2001 Jan 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11154698 11154698]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hi4 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Acharya KR]]
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[[Category: Acharya, K R.]]
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[[Category: Boix E]]
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[[Category: Boix, E.]]
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[[Category: Leonidas DD]]
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[[Category: Leonidas, D D.]]
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[[Category: Minson K]]
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[[Category: Minson, K.]]
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[[Category: Prill R]]
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[[Category: Prill, R.]]
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[[Category: Suzuki M]]
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[[Category: Suzuki, M.]]
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[[Category: Swaminathan GJ]]
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[[Category: Swaminathan, G J.]]
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[[Category: Turton R]]
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[[Category: Turton, R.]]
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[[Category: Youle RJ]]
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[[Category: Youle, R J.]]
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[[Category: Ribonuclease]]
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[[Category: Rnase us]]
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[[Category: Rnase-2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:52:03 2008''
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Current revision

Eosinophil-derived Neurotoxin (EDN) - Adenosien-3'-5'-Diphosphate Complex

PDB ID 1hi4

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