7wex
From Proteopedia
(Difference between revisions)
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==The crystal structure of substrate-free CYP107X1 from Streptomyces avermitilis== | ==The crystal structure of substrate-free CYP107X1 from Streptomyces avermitilis== | ||
| - | <StructureSection load='7wex' size='340' side='right'caption='[[7wex]]' scene=''> | + | <StructureSection load='7wex' size='340' side='right'caption='[[7wex]], [[Resolution|resolution]] 1.99Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WEX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7wex]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avermitilis_MA-4680_=_NBRC_14893 Streptomyces avermitilis MA-4680 = NBRC 14893]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WEX FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wex OCA], [https://pdbe.org/7wex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wex RCSB], [https://www.ebi.ac.uk/pdbsum/7wex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wex ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wex OCA], [https://pdbe.org/7wex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wex RCSB], [https://www.ebi.ac.uk/pdbsum/7wex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wex ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q82A10_STRAW Q82A10_STRAW] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cytochrome P450 enzymes (CYPs or P450s) are ubiquitous heme-dependent enzymes that catalyze the monooxygenation of non-activated C-H bonds to modify the structure of the substrate. In this study, we heterologously expressed CYP107X1 from Streptomyces avermitilis and conducted in vitro substrate screening using the alternative redox partners putidaredoxin and putidaredoxin reductase. CYP107X1 catalyzed the 16alpha-hydroxylation of progesterone with regio- and stereoselectivity. The spectroscopic analyses showed that CYP107X1 bound progesterone with a relatively high K(d) value of 65.3+/-38.9 muM. The K(m) and k(cat) values for progesterone were estimated to be 47.7+/-12.0 muM and 0.30 min(-1) , respectively. Furthermore, a crystal structure was obtained of CYP107X1 bound with glycerol from the buffer solution. Interestingly, a conserved threonine was replaced with asparagine in CYP107X1, indicating that it may adopt an unnatural proton transfer process and play a crucial role in its catalytic activity. | ||
| + | |||
| + | The 16alpha-Hydroxylation of Progesterone by Cytochrome P450 107X1 from Streptomyces avermitilis.,Lin S, Ma B, Gao Q, Yang J, Lai G, Lin R, Yang B, Han BN, Xu LH Chem Biodivers. 2022 May;19(5):e202200177. doi: 10.1002/cbdv.202200177. Epub 2022 , Apr 26. PMID:35426465<ref>PMID:35426465</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7wex" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cytochrome P450 hydroxylase 3D structures|Cytochrome P450 hydroxylase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Streptomyces avermitilis MA-4680 = NBRC 14893]] |
| + | [[Category: Gao L]] | ||
| + | [[Category: Lin S]] | ||
| + | [[Category: Xu L-H]] | ||
Current revision
The crystal structure of substrate-free CYP107X1 from Streptomyces avermitilis
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