7y9y
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the Cas7-11-Csx29-guide RNA-target RNA (no PFS) complex== | |
| + | <StructureSection load='7y9y' size='340' side='right'caption='[[7y9y]], [[Resolution|resolution]] 2.77Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7y9y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y9Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.77Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y9y OCA], [https://pdbe.org/7y9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y9y RCSB], [https://www.ebi.ac.uk/pdbsum/7y9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y9y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A401FT52_9BACT A0A401FT52_9BACT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The type III-E CRISPR-Cas7-11 effector binds a CRISPR RNA (crRNA) and the putative protease Csx29 and catalyzes crRNA-guided RNA cleavage. We report cryo-electron microscopy structures of the Cas7-11-crRNA-Csx29 complex with and without target RNA (tgRNA), and demonstrate that tgRNA binding induces conformational changes in Csx29. Biochemical experiments revealed tgRNA-dependent cleavage of the accessory protein Csx30 by Csx29. Reconstitution of the system in bacteria showed that Csx30 cleavage yields toxic protein fragments that cause growth arrest, which is regulated by Csx31. Csx30 binds Csx31 and the associated sigma factor RpoE (RNA polymerase, extracytoplasmic E), suggesting that Csx30-mediated RpoE inhibition modulates the cellular response to infection. We engineered the Cas7-11-Csx29-Csx30 system for programmable RNA sensing in mammalian cells. Overall, the Cas7-11-Csx29 effector is an RNA-dependent nuclease-protease. | ||
| - | + | RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease.,Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H Science. 2022 Nov 25;378(6622):882-889. doi: 10.1126/science.add7347. Epub 2022 , Nov 3. PMID:36423304<ref>PMID:36423304</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7y9y" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Desulfonema ishimotonii]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Isayama Y]] | ||
| + | [[Category: Ishikawa J]] | ||
| + | [[Category: Kato K]] | ||
| + | [[Category: Nishimasu H]] | ||
| + | [[Category: Nishizawa T]] | ||
| + | [[Category: Okazaki S]] | ||
Current revision
Structure of the Cas7-11-Csx29-guide RNA-target RNA (no PFS) complex
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