1flh
From Proteopedia
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(New page: 200px<br /> <applet load="1flh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1flh, resolution 2.45Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1flh.gif|left|200px]]<br /> | ||
| - | <applet load="1flh" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1flh, resolution 2.45Å" /> | ||
| - | '''CRYSTAL STRUCTURE OF HUMAN UROPEPSIN AT 2.45 A RESOLUTION'''<br /> | ||
| - | == | + | ==CRYSTAL STRUCTURE OF HUMAN UROPEPSIN AT 2.45 A RESOLUTION== |
| - | The molecular structure of human uropepsin, an aspartic proteinase from | + | <StructureSection load='1flh' size='340' side='right'caption='[[1flh]], [[Resolution|resolution]] 2.45Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1flh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FLH FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1flh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1flh OCA], [https://pdbe.org/1flh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1flh RCSB], [https://www.ebi.ac.uk/pdbsum/1flh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1flh ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PEPA4_HUMAN PEPA4_HUMAN] Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/1flh_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1flh ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The molecular structure of human uropepsin, an aspartic proteinase from the urine produced in the form of pepsinogen A in the gastric mucosa, has been determined by molecular replacement using human pepsin as the search model. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.99, b = 75.56, c = 89.90 A. Crystallographic refinement led to an R factor of 0.161 at 2.45 A resolution. The positions of 2437 non-H protein atoms in 326 residues have been determined and the model contains 143 water molecules. The structure is bilobal, consisting of two predominantly beta-sheet lobes which, as observed in other aspartic proteinases, are related by a pseudo-twofold axis. A model of the uropepsin-pepstatin complex has been constructed based on the high-resolution crystal structure of pepsin complexed with pepstatin. | ||
| - | + | Structure of human uropepsin at 2.45 A resolution.,Canduri F, Teodoro LG, Fadel V, Lorenzi CC, Hial V, Gomes RA, Neto JR, de Azevedo WF Jr Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1560-70. Epub 2001, Oct 25. PMID:11679720<ref>PMID:11679720</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1flh" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Pepsin|Pepsin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Canduri F]] | ||
| + | [[Category: De Azevedo Jr WF]] | ||
| + | [[Category: Fadel V]] | ||
| + | [[Category: Gomes RAS]] | ||
| + | [[Category: Hial V]] | ||
| + | [[Category: Lorenzi CCB]] | ||
| + | [[Category: Neto JR]] | ||
| + | [[Category: Teodoro LGVL]] | ||
Current revision
CRYSTAL STRUCTURE OF HUMAN UROPEPSIN AT 2.45 A RESOLUTION
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Categories: Homo sapiens | Large Structures | Canduri F | De Azevedo Jr WF | Fadel V | Gomes RAS | Hial V | Lorenzi CCB | Neto JR | Teodoro LGVL
