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3tqx

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<StructureSection load='3tqx' size='340' side='right'caption='[[3tqx]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='3tqx' size='340' side='right'caption='[[3tqx]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tqx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxbu Coxbu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TQX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tqx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxiella_burnetii_RSA_493 Coxiella burnetii RSA 493]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TQX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.304&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CBU_0111, kbl ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227377 COXBU])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glycine_C-acetyltransferase Glycine C-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.29 2.3.1.29] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tqx OCA], [https://pdbe.org/3tqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tqx RCSB], [https://www.ebi.ac.uk/pdbsum/3tqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tqx ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tqx OCA], [https://pdbe.org/3tqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tqx RCSB], [https://www.ebi.ac.uk/pdbsum/3tqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tqx ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/Q83F40_COXBU Q83F40_COXBU]] Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA.[HAMAP-Rule:MF_00985]
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[https://www.uniprot.org/uniprot/Q83F40_COXBU Q83F40_COXBU] Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA.[HAMAP-Rule:MF_00985]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Coxiella burnetii is a highly infectious bacterium and potential agent of bioterrorism. However, it has not been studied as extensively as other biological agents, and very few of its proteins have been structurally characterized. To address this situation, we undertook a study of critical metabolic enzymes in C. burnetii that have great potential as drug targets. We used high-throughput techniques to produce novel crystal structures of 48 of these proteins. We selected one protein, C. burnetii dihydrofolate reductase (CbDHFR), for additional work to demonstrate the value of these structures for structure-based drug design. This enzyme's structure reveals a feature in the substrate binding groove that is different between CbDHFR and human dihydrofolate reductase (hDFHR). We then identified a compound by in silico screening that exploits this binding groove difference, and demonstrated that this compound inhibits CbDHFR with at least 25-fold greater potency than hDHFR. Since this binding groove feature is shared by many other prokaryotes, the compound identified could form the basis of a novel antibacterial agent effective against a broad spectrum of pathogenic bacteria. This article is protected by copyright. All rights reserved.
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Structural Genomics for Drug Design against the Pathogen Coxiella burnetii.,Franklin MC, Cheung J, Rudolph MJ, Burshteyn F, Cassidy M, Gary E, Hillerich B, Yao ZK, Carlier PR, Totrov M, Love JD Proteins. 2015 Jun 1. doi: 10.1002/prot.24841. PMID:26033498<ref>PMID:26033498</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3tqx" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Coxbu]]
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[[Category: Coxiella burnetii RSA 493]]
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[[Category: Glycine C-acetyltransferase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Burshteyn, F]]
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[[Category: Burshteyn F]]
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[[Category: Cassidy, M]]
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[[Category: Cassidy M]]
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[[Category: Cheung, J]]
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[[Category: Cheung J]]
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[[Category: Franklin, M C]]
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[[Category: Franklin MC]]
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[[Category: Gary, E]]
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[[Category: Gary E]]
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[[Category: Love, J]]
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[[Category: Love J]]
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[[Category: Rudolph, M]]
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[[Category: Rudolph M]]
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[[Category: Energy metabolism]]
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[[Category: Transferase]]
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Current revision

Structure of the 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Coxiella burnetii

PDB ID 3tqx

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