7ub2
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of RecT protein from Listeria innoccua phage A118 in complex with 83-mer annealed duplex== | |
| + | <StructureSection load='7ub2' size='340' side='right'caption='[[7ub2]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7ub2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_M13 Escherichia virus M13] and [https://en.wikipedia.org/wiki/Listeria_innocua_Clip11262 Listeria innocua Clip11262]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UB2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ub2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ub2 OCA], [https://pdbe.org/7ub2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ub2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ub2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ub2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q92FL9_LISIN Q92FL9_LISIN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Some bacteriophage encode a recombinase that catalyzes single-stranded DNA annealing (SSA). These proteins are apparently related to RAD52, the primary human SSA protein. The best studied protein, Redbeta from bacteriophage lambda, binds weakly to ssDNA, not at all to dsDNA, but tightly to a duplex intermediate of annealing formed when two complementary DNA strands are added to the protein sequentially. We used single particle cryo-electron microscopy (cryo-EM) to determine a 3.4 A structure of a Redbeta homolog from a prophage of Listeria innocua in complex with two complementary 83mer oligonucleotides. The structure reveals a helical protein filament bound to a DNA duplex that is highly extended and unwound. Native mass spectrometry confirms that the complex seen by cryo-EM is the predominant species in solution. The protein shares a common core fold with RAD52 and a similar mode of ssDNA-binding. These data provide insights into the mechanism of protein-catalyzed SSA. | ||
| - | + | Structure of a RecT/Redbeta family recombinase in complex with a duplex intermediate of DNA annealing.,Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE Nat Commun. 2022 Dec 21;13(1):7855. doi: 10.1038/s41467-022-35572-z. PMID:36543802<ref>PMID:36543802</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7ub2" style="background-color:#fffaf0;"></div> |
| - | [[Category: Bell | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia virus M13]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Listeria innocua Clip11262]] | ||
| + | [[Category: Bell CE]] | ||
| + | [[Category: Caldwell BJ]] | ||
Current revision
Structure of RecT protein from Listeria innoccua phage A118 in complex with 83-mer annealed duplex
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