8a3b
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of mouse Pannexin 1 purified in Salipro nanoparticles== | |
| + | <StructureSection load='8a3b' size='340' side='right'caption='[[8a3b]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8a3b]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A3B FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a3b OCA], [https://pdbe.org/8a3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a3b RCSB], [https://www.ebi.ac.uk/pdbsum/8a3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a3b ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PANX1_MOUSE PANX1_MOUSE] Ion channel involved in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and oogenesis (PubMed:30814251). Forms anion-selective channels with relatively low conductance and an order of permeabilities: nitrate>iodide>chlroride>>aspartate=glutamate=gluconate (PubMed:22311122). Can release ATP upon activation through phosphorylation or cleavage at C-terminus (PubMed:32238926). May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis (By similarity).[UniProtKB:Q96RD7]<ref>PMID:22311122</ref> <ref>PMID:30814251</ref> <ref>PMID:32238926</ref> During apoptosis and after cleavage by caspases of the C-terminal tail, acts as a plasma membrane channel which mediates the regulated release of find-me signals, such as nucleotides ATP and UTP, and selective plasme membrane permeability.<ref>PMID:32238926</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM. | ||
| - | + | Direct cell extraction of membrane proteins for structure-function analysis.,Drulyte I, Gutgsell AR, Lloris-Garcera P, Liss M, Geschwindner S, Radjainia M, Frauenfeld J, Loving R Sci Rep. 2023 Jan 25;13(1):1420. doi: 10.1038/s41598-023-28455-w. PMID:36697499<ref>PMID:36697499</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Drulyte | + | <div class="pdbe-citations 8a3b" style="background-color:#fffaf0;"></div> |
| + | |||
| + | ==See Also== | ||
| + | *[[Pannexin|Pannexin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Drulyte I]] | ||
Current revision
Cryo-EM structure of mouse Pannexin 1 purified in Salipro nanoparticles
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