3tsj

From Proteopedia

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Current revision

Crystal structure of Phl p 4, a grass pollen allergen with glucose dehydrogenase activity

Structural highlights

3tsj is a 2 chain structure with sequence from Phleum pratense. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q2I6V7_PHLPR

Publication Abstract from PubMed

BACKGROUND: Phl p 4 is a major pollen allergen but exhibits lower allergenicity than other major allergens. The natural protein is glycosylated and shows cross-reactivity with related and structurally unrelated allergens. OBJECTIVE: We sought to determine the high-resolution crystal structure of Phl p 4 and to evaluate the immunologic properties of the recombinant allergen in comparison with natural Phl p 4. METHODS: Different isoallergens of Phl p 4 were expressed, and the nonglycosylated mutant was crystallized. The specific role of protein and carbohydrate epitopes for allergenicity was studied by using IgE inhibition and basophil release assays. RESULTS: The 3-dimensional structure was determined by using x-ray crystallography at a resolution of 1.9 A. The allergen is a glucose dehydrogenase with a bicovalently attached flavin adenine dinucleotide. Glycosylated and nonglycosylated recombinant Phl p 4 showed identical inhibition of IgE binding, but compared with natural Phl p 4, all recombinant isoforms displayed a reduced IgE-binding inhibition. However, the recombinant protein exhibited an approximately 10-fold higher potency in basophil release assays than the natural protein. CONCLUSION: The crystal structure reveals the compact globular nature of the protein, and the observed binding pocket implies the size of the natural substrate. Plant-derived cross-reactive carbohydrate determinants (CCDs) appear to reduce the allergenicity of the natural allergen, whereas the Pichia pastoris-derived glycosylation does not. Our results imply yet undescribed mechanism of how CCDs dampen the immune response, leading to a novel understanding of the role of CCDs.

Crystal structure and immunologic characterization of the major grass pollen allergen Phl p 4.,Zafred D, Nandy A, Pump L, Kahlert H, Keller W J Allergy Clin Immunol. 2013 Sep;132(3):696-703.e10. doi:, 10.1016/j.jaci.2013.03.021. Epub 2013 May 14. PMID:23683465^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zafred D, Nandy A, Pump L, Kahlert H, Keller W. Crystal structure and immunologic characterization of the major grass pollen allergen Phl p 4. J Allergy Clin Immunol. 2013 Sep;132(3):696-703.e10. doi:, 10.1016/j.jaci.2013.03.021. Epub 2013 May 14. PMID:23683465 doi:http://dx.doi.org/10.1016/j.jaci.2013.03.021

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3tsj"

Categories: Large Structures | Phleum pratense | Keller W | Nandy A | Zafred D

@@ Line 3: / Line 3: @@
 <StructureSection load='3tsj' size='340' side='right'caption='[[3tsj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3tsj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TSJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3tsj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TSJ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3q3r|3q3r]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsj OCA], [https://pdbe.org/3tsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tsj RCSB], [https://www.ebi.ac.uk/pdbsum/3tsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tsj ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2I6V7_PHLPR Q2I6V7_PHLPR]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Keller, W]]
+[[Category: Phleum pratense]]
-[[Category: Nandy, A]]
+[[Category: Keller W]]
-[[Category: Zafred, D]]
+[[Category: Nandy A]]
-[[Category: Allergen]]
+[[Category: Zafred D]]
-[[Category: Allergy]]
-[[Category: Bi-covalent flavinylation]]
-[[Category: Dehydrogenase]]
-[[Category: Flavoprotein]]
-[[Category: Glucose dehydrogenase]]
-[[Category: Grass pollen]]
-[[Category: N-glycosylation]]
-[[Category: Oxidoreductase]]
-[[Category: Pollen]]

3tsj

From Proteopedia

Current revision

Crystal structure of Phl p 4, a grass pollen allergen with glucose dehydrogenase activity

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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