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1foe

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(New page: 200px<br /> <applet load="1foe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1foe, resolution 2.8&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1foe.gif|left|200px]]<br />
 
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<applet load="1foe" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1foe, resolution 2.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RAC1 IN COMPLEX WITH THE GUANINE NUCLEOTIDE EXCHANGE REGION OF TIAM1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RAC1 IN COMPLEX WITH THE GUANINE NUCLEOTIDE EXCHANGE REGION OF TIAM1==
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The principal guanine nucleotide exchange factors for Rho family G, proteins contain tandem Dbl-homology (DH) and pleckstrin-homology (PH), domains that catalyse nucleotide exchange and the activation of G, proteins. Here we have determined the crystal structure of the DH and PH, domains of the T-lymphoma invasion and metastasis factor 1 (Tiam1) protein, in complex with its cognate Rho family G protein, Rac1. The two switch, regions of Rac1 are stabilized in conformations that disrupt both, magnesium binding and guanine nucleotide interaction. The resulting cleft, in Rac1 is devoid of nucleotide and highly exposed to solvent. The PH, domain of Tiam1 does not contact Rac1, and the position and orientation of, the PH domain is markedly altered relative to the structure of the, uncomplexed, GTPase-free DH/PH element from Sos1. The Tiam1/Rac1 structure, highlights the interactions that catalyse nucleotide exchange on Rho, family G proteins, and illustrates structural determinants dictating, specificity between individual Rho family members and their associated, Dbl-related guanine nucleotide exchange factors.
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<StructureSection load='1foe' size='340' side='right'caption='[[1foe]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1foe]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FOE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1foe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1foe OCA], [https://pdbe.org/1foe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1foe RCSB], [https://www.ebi.ac.uk/pdbsum/1foe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1foe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TIAM1_MOUSE TIAM1_MOUSE] Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA (By similarity). Affects invasiveness of T-lymphoma cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/1foe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1foe ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FOE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FOE OCA].
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*[[Rac 3D structures|Rac 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1., Worthylake DK, Rossman KL, Sondek J, Nature. 2000 Dec 7;408(6813):682-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11130063 11130063]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Rossman KL]]
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[[Category: Rossman, K.L.]]
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[[Category: Sondek J]]
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[[Category: Sondek, J.]]
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[[Category: Worthylake DK]]
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[[Category: Worthylake, D.K.]]
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[[Category: SO4]]
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[[Category: dbl homology domain]]
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[[Category: gtpase]]
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[[Category: guanine nucleotide exchange factor]]
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[[Category: pleckstrin homology domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:55:46 2007''
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Current revision

CRYSTAL STRUCTURE OF RAC1 IN COMPLEX WITH THE GUANINE NUCLEOTIDE EXCHANGE REGION OF TIAM1

PDB ID 1foe

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