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3u75

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Structure of E230A-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosylnystose

Structural highlights

3u75 is a 4 chain structure with sequence from Schwanniomyces occidentalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.68Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

E5D0X5_SCHOC

Publication Abstract from PubMed

Schwanniomyces occidentalis beta-fructofuranosidase (Ffase) is a GH32 dimeric enzyme that releases fructose from the nonreducing end of various oligosaccharides and essential storage fructans such as inulin. It also catalyzes the transfer of a fructosyl unit to an acceptor producing 6-kestose and 1-kestose, prebiotics that stimulate the growth of bacteria beneficial for human health. We report here the crystal structure of inactivated Ffase complexed with fructosylnystose and inulin, which shows the intricate net of interactions keeping the substrate tightly bound at the active site. Up to five subsites are observed, the sugar unit located at subsite +3 being recognized by interaction with the beta-sandwich domain of the adjacent subunit within the dimer. This explains the high activity observed against long substrates and gives the first experimental evidence of the direct role of a GH32 beta-sandwich domain in substrate binding. Crucial residues were mutated and their hydrolase/transferase (H/T) activities were fully characterized, showing the involvement of the Gln228/Asn254 pair in modulating the H/T ratio and the type beta(2-1)/beta(2-6) linkage formation. We have generated Ffase mutants with new transferase activity; among them, Q228V gives almost specifically 6-kestose while N254T produces a broader spectrum product including also neokestose. A model for the mechanism of the Ffase transfructosylation reaction has been proposed. The results contribute to understand the molecular basis regulating specificity among GH-J clan members, which represent an interesting target for rational design of enzymes showing redesigned activities to produce tailor-made FOS.

Structural and kinetic insights reveal that the amino acid pair GLN228/ASN254 modulates the transfructosylating specificity of Schwanniomyces occidentalis beta-fructofuranosidase, an enzyme that produces prebiotics.,Alvaro-Benito M, Sainz-Polo MA, Gonzalez-Perez D, Gonzalez B, Plou FJ, Fernandez-Lobato M, Sanz Aparicio J J Biol Chem. 2012 Apr 18. PMID:22511773^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Alvaro-Benito M, Sainz-Polo MA, Gonzalez-Perez D, Gonzalez B, Plou FJ, Fernandez-Lobato M, Sanz Aparicio J. Structural and kinetic insights reveal that the amino acid pair GLN228/ASN254 modulates the transfructosylating specificity of Schwanniomyces occidentalis beta-fructofuranosidase, an enzyme that produces prebiotics. J Biol Chem. 2012 Apr 18. PMID:22511773 doi:10.1074/jbc.M112.355503

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3u75"

Categories: Large Structures | Schwanniomyces occidentalis | Sainz-Polo MA | Sanz-Aparicio J

@@ Line 3: / Line 3: @@
 <StructureSection load='3u75' size='340' side='right'caption='[[3u75]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3u75]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_2322 Atcc 2322]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U75 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3u75]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schwanniomyces_occidentalis Schwanniomyces occidentalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U75 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3kf3|3kf3]], [[3kf5|3kf5]], [[3u14|3u14]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u75 OCA], [https://pdbe.org/3u75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u75 RCSB], [https://www.ebi.ac.uk/pdbsum/3u75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u75 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/E5D0X5_SCHOC E5D0X5_SCHOC]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 2322]]
-[[Category: Beta-fructofuranosidase]]
 [[Category: Large Structures]]
-[[Category: Sainz-Polo, M A]]
+[[Category: Schwanniomyces occidentalis]]
-[[Category: Sanz-Aparicio, J]]
+[[Category: Sainz-Polo MA]]
-[[Category: Beta-propeller]]
+[[Category: Sanz-Aparicio J]]
-[[Category: Carbohydrate/sugar binding]]
-[[Category: Glycosidase gh32]]
-[[Category: Glycosylation]]
-[[Category: Hydrolase]]

3u75

From Proteopedia

Current revision

Structure of E230A-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosylnystose

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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