7yfz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7yfz is ON HOLD Authors: Yang, F., Jiang, Y.L., Zhou, C.Z. Description: Cyanophage Pam3 baseplate proteins Category: Unreleased Structures [[Ca...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Cyanophage Pam3 baseplate proteins== | |
| + | <StructureSection load='7yfz' size='340' side='right'caption='[[7yfz]], [[Resolution|resolution]] 3.19Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7yfz]] is a 42 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_cyanophage Uncultured cyanophage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YFZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.19Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yfz OCA], [https://pdbe.org/7yfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yfz RCSB], [https://www.ebi.ac.uk/pdbsum/7yfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yfz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A9E7DTG9_9CAUD A0A9E7DTG9_9CAUD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater Myoviridae cyanophage Pam3, which has an icosahedral capsid of ~680 A in diameter, connected via a three-section neck to an 840-A-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology. | ||
| - | + | Fine structure and assembly pattern of a minimal myophage Pam3.,Yang F, Jiang YL, Zhang JT, Zhu J, Du K, Yu RC, Wei ZL, Kong WW, Cui N, Li WF, Chen Y, Li Q, Zhou CZ Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2213727120. doi: , 10.1073/pnas.2213727120. Epub 2023 Jan 19. PMID:36656854<ref>PMID:36656854</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Jiang | + | <div class="pdbe-citations 7yfz" style="background-color:#fffaf0;"></div> |
| - | [[Category: Yang | + | == References == |
| - | [[Category: Zhou | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Uncultured cyanophage]] | ||
| + | [[Category: Jiang YL]] | ||
| + | [[Category: Yang F]] | ||
| + | [[Category: Zhou CZ]] | ||
Current revision
Cyanophage Pam3 baseplate proteins
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