7w58

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<StructureSection load='7w58' size='340' side='right'caption='[[7w58]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
<StructureSection load='7w58' size='340' side='right'caption='[[7w58]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7w58]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W58 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7w58]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W58 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Holo-[acyl-carrier-protein]_synthase Holo-[acyl-carrier-protein] synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.8.7 2.7.8.7] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w58 OCA], [https://pdbe.org/7w58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w58 RCSB], [https://www.ebi.ac.uk/pdbsum/7w58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w58 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w58 OCA], [https://pdbe.org/7w58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w58 RCSB], [https://www.ebi.ac.uk/pdbsum/7w58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w58 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/A0A8B4R1L0_MYCSM A0A8B4R1L0_MYCSM]] Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.[HAMAP-Rule:MF_00101]
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[https://www.uniprot.org/uniprot/A0A8B4R1L0_MYCSM A0A8B4R1L0_MYCSM] Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.[HAMAP-Rule:MF_00101]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The unintended crystallization of proteins which generally originate from the expression host instead of the target recombinant proteins is periodically reported. Despite the massive technological advances in the field, assigning a structural model to the corresponding diffraction data is not a trivial task. Here, the structure of acyl-carrier protein synthase (AcpS) from Mycobacterium smegmatis (msAcpS), which crystallized inadvertently in an experimental setup to grow crystals of a Mycobacterium tuberculosis protein using M. smegmatis as an expression system, is reported. After numerous unsuccessful attempts to solve the structure of the target protein by the molecular-replacement method no convincing solutions were obtained, indicating that the diffraction data may correspond to a crystal of an artifactual protein, which was finally identified by the Sequence-Independent Molecular replacement Based on Available Databases (SIMBAD) server. The msAcpS structure was solved at 2.27 A resolution and structural analysis showed an overall conserved fold. msAcpS formed a trimeric structure similar to those of other reported structures of AcpS from various organisms; however, the residues involved in trimer formation are not strictly conserved. An unrelated metal ion (Ni(2+)), which was possibly incorporated during protein purification, was observed in the proximity of His49 and His116. Structural and sequence differences were observed in the loop connecting the alpha3 and alpha4 helices that is responsible for the open and closed conformations of the enzyme. Moreover, the structural analysis of msAcpS augments the current understanding of this enzyme, which plays a crucial role in the functional activation of acyl-carrier proteins in the fatty-acid biosynthesis pathway.
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Identification, structure determination and analysis of Mycobacterium smegmatis acyl-carrier protein synthase (AcpS) crystallized serendipitously.,Bhatia I, Yadav S, Biswal BK Acta Crystallogr F Struct Biol Commun. 2022 Jul 1;78(Pt 7):252-264. doi:, 10.1107/S2053230X22005738. Epub 2022 Jun 15. PMID:35787552<ref>PMID:35787552</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7w58" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Bhatia, I]]
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[[Category: Mycolicibacterium smegmatis]]
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[[Category: Biswal, B K]]
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[[Category: Bhatia I]]
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[[Category: Yadav, S]]
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[[Category: Biswal BK]]
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[[Category: Acp]]
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[[Category: Yadav S]]
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[[Category: Mycobacterium smegmati]]
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[[Category: Transferase]]
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Current revision

Crystal structure of acyl-carrier protein synthase from Mycobacterium smegmatis

PDB ID 7w58

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