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3uno

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Mycobacterium tuberculosis ferritin homolog, BfrB

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Retrieved from "http://52.214.119.220/wiki/index.php/3uno"

Categories: Large Structures | Mycobacterium tuberculosis | Contreras H | Goulding CW | McMath LM

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 <StructureSection load='3uno' size='340' side='right'caption='[[3uno]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3uno]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UNO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3uno]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UNO FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3oj5|3oj5]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bfrB, ftn, MT3949 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uno OCA], [https://pdbe.org/3uno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uno RCSB], [https://www.ebi.ac.uk/pdbsum/3uno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uno ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/BFRB_MYCTU BFRB_MYCTU]] Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe(2+) ions into Fe(3+) ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.<ref>PMID:21494619</ref>
+[https://www.uniprot.org/uniprot/BFRB_MYCTU BFRB_MYCTU] Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe(2+) ions into Fe(3+) ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.<ref>PMID:21494619</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Mycobacterium tuberculosis (Mtb) is the causative agent of the deadly disease tuberculosis. Iron acquisition, regulation and storage are critical for the survival of this pathogen within a host. Thus, understanding the mechanisms of iron metabolism in Mtb will shed light on its pathogenic nature, as iron is important for infection. Ferritins are a superfamily of protein nanocages that function in both iron detoxification and storage, and Mtb contains both a predicted ferritin and a bacterioferritin. Here, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the ferritin homolog (Mtb BfrB, Rv3841) is reported. An Mtb BfrB crystal grown at pH 6.5 using the hanging-drop vapor-diffusion technique diffracted to 2.50 A resolution and belonged to space group C2, with unit-cell parameters a=226.2, b=226.8, c=113.7 A, beta=94.7 degrees and with 24 subunits per asymmetric unit. Furthermore, modeling the crystal structure of a homologous ferritin into a low-resolution small-angle X-ray scattering (SAXS) electron-density envelope is consistent with the presence of 24 subunits in the BfrB protein cage quaternary structure.
-Crystallization and preliminary X-ray crystallographic analysis of a Mycobacterium tuberculosis ferritin homolog, BfrB.,McMath LM, Habel JE, Sankaran B, Yu M, Hung LW, Goulding CW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1657-61., doi: 10.1107/S1744309110042958. Epub 2010 Nov 26. PMID:21139218<ref>PMID:21139218</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3uno" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 24: / Line 14: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Contreras, H]]
+[[Category: Mycobacterium tuberculosis]]
-[[Category: Goulding, C W]]
+[[Category: Contreras H]]
-[[Category: McMath, L M]]
+[[Category: Goulding CW]]
-[[Category: Structural genomic]]
+[[Category: McMath LM]]
-[[Category: Ferritin]]
-[[Category: Ferroxidase activity]]
-[[Category: Iron storage]]
-[[Category: Oxidoreductase]]
-[[Category: Tbsgc]]

3uno

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Mycobacterium tuberculosis ferritin homolog, BfrB

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