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| | <StructureSection load='3ur7' size='340' side='right'caption='[[3ur7]], [[Resolution|resolution]] 1.40Å' scene=''> | | <StructureSection load='3ur7' size='340' side='right'caption='[[3ur7]], [[Resolution|resolution]] 1.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Potato Potato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UR7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UR7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ghs|1ghs]], [[2cyg|2cyg]], [[3em5|3em5]], [[3f55|3f55]], [[3ur8|3ur8]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato])</td></tr> | + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [https://pdbe.org/3ur7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ur7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur7 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [https://pdbe.org/3ur7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ur7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q70C53_SOLTU Q70C53_SOLTU] |
| - | Endo-1,3-beta-glucanases are widely distributed among bacteria, fungi and higher plants. They are responsible for hydrolysis of the glycosidic bond in specific polysaccharides with tracts of unsubstituted beta-1,3-linked glucosyl residues. The plant enzymes belong to glycoside hydrolase family 17 (GH17) and are also members of class 2 of pathogenesis-related (PR) proteins. X-ray diffraction data were collected to 1.40 and 1.26 A resolution from two crystals of endo-1,3-beta-glucanase from Solanum tuberosum (potato, cultivar Desiree) which, despite having a similar packing framework, represented two separate crystal forms. In particular, they differed in the Matthews coefficient and are consequently referred to as higher density (HD; 1.40 A resolution) and lower density (LD; 1.26 A resolution) forms. The general fold of the protein resembles that of other known plant endo-1,3-beta-glucanases and is defined by a (beta/alpha)(8)-barrel with an additional subdomain built around the C-terminal half of the barrel. The structures revealed high flexibility of the subdomain, which forms part of the catalytic cleft. Comparison with structures of other GH17 endo-1,3-beta-glucanases revealed differences in the arrangement of the secondary-structure elements in this region, which can be correlated with sequence variability and may suggest distinct substrate-binding patterns. The crystal structures revealed an unusual packing mode, clearly visible in the LD structure, caused by the presence of the C-terminal His(6) tag, which extends from the compact fold of the enzyme molecule and docks in the catalytic cleft of a neighbouring molecule. In this way, an infinite chain of His-tag-linked protein molecules is formed along the c direction.
| + | |
| - | | + | |
| - | Two high-resolution structures of potato endo-1,3-beta-glucanase reveal subdomain flexibility with implications for substrate binding.,Wojtkowiak A, Witek K, Hennig J, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):713-23. doi:, 10.1107/S090744491200995X. Epub 2012 May 17. PMID:22683794<ref>PMID:22683794</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3ur7" style="background-color:#fffaf0;"></div>
| + | |
| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] | | *[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] |
| | *[[Glucanase 3D structures|Glucanase 3D structures]] | | *[[Glucanase 3D structures|Glucanase 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Glucan endo-1,3-beta-D-glucosidase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Potato]] | + | [[Category: Solanum tuberosum]] |
| - | [[Category: Hennig, J]] | + | [[Category: Hennig J]] |
| - | [[Category: Jaskolski, M]] | + | [[Category: Jaskolski M]] |
| - | [[Category: Witek, K]] | + | [[Category: Witek K]] |
| - | [[Category: Wojtkowiak, A]] | + | [[Category: Wojtkowiak A]] |
| - | [[Category: Carbohydrate/sugar binding]]
| + | |
| - | [[Category: Gh17 family]]
| + | |
| - | [[Category: Glucoside hydrolase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Tim barrel]]
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