7ff0
From Proteopedia
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==Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and auranofin== | ==Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and auranofin== | ||
| - | <StructureSection load='7ff0' size='340' side='right'caption='[[7ff0]]' scene=''> | + | <StructureSection load='7ff0' size='340' side='right'caption='[[7ff0]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FF0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ff0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FF0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ff0 OCA], [https://pdbe.org/7ff0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ff0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ff0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ff0 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene>, <scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ff0 OCA], [https://pdbe.org/7ff0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ff0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ff0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ff0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/VFR_PSEAE VFR_PSEAE] Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pseudomonas aeruginosa is a leading cause of hospital-acquired infections. Treatment of P. aeruginosa infections is difficult given its multiple virulence mechanisms, intrinsic antibiotic resistance mechanisms, and biofilm-forming ability. Auranofin, an approved oral gold compound for rheumatoid arthritis treatment, was recently reported to inhibit the growth of multiple bacterial species. Here, we identify P. aeruginosa's global virulence factor regulator Vfr as one target of auranofin. We report the mechanistic insights into the inhibitory mechanism of auranofin and gold(I) analogues to Vfr through structural, biophysical, and phenotypic inhibition studies. This work suggests that auranofin and gold(I) analogues have potential to be developed as anti-virulence drugs against P. aeruginosa. | ||
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| + | Structural basis for the inhibitory mechanism of auranofin and gold(I) analogues against Pseudomonas aeruginosa global virulence factor regulator Vfr.,Zhang Y, Chew BLA, Wang J, Yuan M, Yam JKH, Luo D, Yang L Comput Struct Biotechnol J. 2023 Mar 13;21:2137-2146. doi: , 10.1016/j.csbj.2023.03.013. eCollection 2023. PMID:37007650<ref>PMID:37007650</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ff0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Chew BLA]] | [[Category: Chew BLA]] | ||
[[Category: Luo D]] | [[Category: Luo D]] | ||
Current revision
Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and auranofin
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