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| | <StructureSection load='3wc4' size='340' side='right'caption='[[3wc4]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='3wc4' size='340' side='right'caption='[[3wc4]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wc4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Butterfly_pea Butterfly pea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WC4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wc4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clitoria_ternatea Clitoria ternatea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WC4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ct3GT-A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43366 Butterfly pea])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Anthocyanidin_3-O-glucosyltransferase Anthocyanidin 3-O-glucosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.115 2.4.1.115] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wc4 OCA], [https://pdbe.org/3wc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wc4 RCSB], [https://www.ebi.ac.uk/pdbsum/3wc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wc4 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wc4 OCA], [https://pdbe.org/3wc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wc4 RCSB], [https://www.ebi.ac.uk/pdbsum/3wc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wc4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A4F1R4_CLITE A4F1R4_CLITE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anthocyanidin 3-O-glucosyltransferase]] | + | [[Category: Clitoria ternatea]] |
| - | [[Category: Butterfly pea]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hiromoto, T]] | + | [[Category: Hiromoto T]] |
| - | [[Category: Honjo, E]] | + | [[Category: Honjo E]] |
| - | [[Category: Kuroki, R]] | + | [[Category: Kuroki R]] |
| - | [[Category: Tamada, T]] | + | [[Category: Tamada T]] |
| - | [[Category: Glucosylation]]
| + | |
| - | [[Category: Glucosyltransferase]]
| + | |
| - | [[Category: Gt-b fold]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Udp-glucose/anthocyanidin binding]]
| + | |
| Structural highlights
Function
A4F1R4_CLITE
Publication Abstract from PubMed
Flowers of the butterfly pea (Clitoria ternatea) accumulate a group of polyacylated anthocyanins, named ternatins, in their petals. The first step in ternatin biosynthesis is the transfer of glucose from UDP-glucose to anthocyanidins such as delphinidin, a reaction catalyzed in C. ternatea by UDP-glucose:anthocyanidin 3-O-glucosyltransferase (Ct3GT-A; AB185904). To elucidate the structure-function relationship of Ct3GT-A, recombinant Ct3GT-A was expressed in Escherichia coli and its tertiary structure was determined to 1.85 A resolution by using X-ray crystallography. The structure of Ct3GT-A shows a common folding topology, the GT-B fold, comprised of two Rossmann-like beta/alpha/beta domains and a cleft located between the N- and C-domains containing two cavities that are used as binding sites for the donor (UDP-Glc) and acceptor substrates. By comparing the structure of Ct3GT-A with that of the flavonoid glycosyltransferase VvGT1 from red grape (Vitis vinifera) in complex with UDP-2-deoxy-2-fluoro glucose and kaempferol, locations of the catalytic His-Asp dyad and the residues involved in recognizing UDP-2-deoxy-2-fluoro glucose were essentially identical in Ct3GT-A, but certain residues of VvGT1 involved in binding kaempferol were found to be substituted in Ct3GT-A. These findings are important for understanding the differentiation of acceptor-substrate recognition in these two enzymes.
Crystal structure of UDP-glucose:anthocyanidin 3-O-glucosyltransferase from Clitoria ternatea.,Hiromoto T, Honjo E, Tamada T, Noda N, Kazuma K, Suzuki M, Kuroki R J Synchrotron Radiat. 2013 Nov;20(Pt 6):894-8. doi: 10.1107/S0909049513020712., Epub 2013 Sep 29. PMID:24121335[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hiromoto T, Honjo E, Tamada T, Noda N, Kazuma K, Suzuki M, Kuroki R. Crystal structure of UDP-glucose:anthocyanidin 3-O-glucosyltransferase from Clitoria ternatea. J Synchrotron Radiat. 2013 Nov;20(Pt 6):894-8. doi: 10.1107/S0909049513020712., Epub 2013 Sep 29. PMID:24121335 doi:http://dx.doi.org/10.1107/S0909049513020712
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