Flocculation protein
From Proteopedia
(Difference between revisions)
| (5 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | <StructureSection load='2xjp' size='340' side='right' caption='FLO5 N-terminal domain complex with α-mannose, β-mannose, Ca+2 (large green), Cl- (small green) and K+ (purple) ions (PDB ID [[2xjp]])' scene=''> | + | <StructureSection load='2xjp' size='340' side='right' caption='FLO5 N-terminal domain complex with α-mannose, β-mannose, Ca+2 (large green), Cl- (small green) and K+ (purple) ions (PDB ID [[2xjp]])' scene='91/919029/Cv/1'> |
== Function == | == Function == | ||
| - | '''Flocculation protein''' or''' flocculin''' (FLO) are yeast adhesion protein family involved in cell-cell adhesion via lectin-carbohydrate interaction<ref>PMID:20640875</ref>. | + | '''Flocculation protein''' or''' flocculin''' (FLO) are yeast adhesion protein family involved in cell-cell adhesion via lectin-carbohydrate interaction<ref>PMID:20640875</ref>. '''FLO1, FLO5, FLO9 and FLO10''' have similar sequence and cause formation of cellular clumps (flocs) while '''FLO11''' has unrelated sequence and is required for diploid formation and haploid invasive growth. Flocculins confer calcium-dependent cell-cell adhesion, formation of cellular flocs and protection against environmental stress. |
== Relevance == | == Relevance == | ||
| Line 11: | Line 11: | ||
== Structural highlights == | == Structural highlights == | ||
| - | The N-terminal domain of FLO is similar to the lectin-like domain of [[Adhesin]]. | + | The N-terminal domain of FLO is similar to the lectin-like domain of [[Adhesin]]. The Ca+2 ion binds to two carbohydrate-binding groups: a D''cis''D motif - 2 Asp residues connected with a ''cis'' bond and another β strand residues. The Ca+2 ion interacts with α-mannose which is an antiflocculant in vivo<ref>PMID:21149680</ref>. <scene name='91/919029/Cv/7'>α-mannose binding site</scene>. |
==3D structures of flocculation protein== | ==3D structures of flocculation protein== | ||
[[Flocculation protein 3D structures]] | [[Flocculation protein 3D structures]] | ||
Current revision
| |||||||||||
References
- ↑ Goossens K, Willaert R. Flocculation protein structure and cell-cell adhesion mechanism in Saccharomyces cerevisiae. Biotechnol Lett. 2010 Nov;32(11):1571-85. doi: 10.1007/s10529-010-0352-3. Epub, 2010 Jul 18. PMID:20640875 doi:http://dx.doi.org/10.1007/s10529-010-0352-3
- ↑ Verstrepen KJ, Derdelinckx G, Verachtert H, Delvaux FR. Yeast flocculation: what brewers should know. Appl Microbiol Biotechnol. 2003 May;61(3):197-205. doi:, 10.1007/s00253-002-1200-8. Epub 2003 Jan 25. PMID:12698276 doi:http://dx.doi.org/10.1007/s00253-002-1200-8
- ↑ Veelders M, Bruckner S, Ott D, Unverzagt C, Mosch HU, Essen LO. Structural basis of flocculin-mediated social behavior in yeast. Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22511-6. Epub 2010 Dec 13. PMID:21149680 doi:10.1073/pnas.1013210108
