7ynb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "7ynb" [edit=sysop:move=sysop])
Current revision (07:33, 3 July 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 7ynb is ON HOLD
+
==Cryo-EM structure of Cas7-11-crRNA bound to target RNA-2==
 +
<StructureSection load='7ynb' size='340' side='right'caption='[[7ynb]], [[Resolution|resolution]] 3.46&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[7ynb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YNB FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.46&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ynb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ynb OCA], [https://pdbe.org/7ynb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ynb RCSB], [https://www.ebi.ac.uk/pdbsum/7ynb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ynb ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/A0A401FT36_9BACT A0A401FT36_9BACT]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The recently discovered type III-E CRISPR-Cas effector Cas7-11 shows promise when used as an RNA manipulation tool, but its structure and the mechanisms underlying its function remain unclear. Here we present four cryo-EM structures of Desulfonema ishimotonii Cas7-11-crRNA complex in pre-target and target RNA-bound states, and the cryo-EM structure of DiCas7-11-crRNA bound to its accessory protein DiCsx29. These data reveal structural elements for pre-crRNA processing, target RNA cleavage and regulation. Moreover, a 3' seed region of crRNA is involved in regulating RNA cleavage activity of DiCas7-11-crRNA-Csx29. Our analysis also shows that both the minimal mismatch of 4 nt to the 5' handle of crRNA and the minimal matching of the first 12 nt of the spacer by the target RNA are essential for triggering the protease activity of DiCas7-11-crRNA-Csx29 towards DiCsx30. Taken together, we propose that target RNA recognition and cleavage regulate and fine-tune the protease activity of DiCas7-11-crRNA-Csx29, thus preventing auto-immune responses.
-
Authors: Yangao, H., Qinghua, D., Hongshen, Z., Tao, J.
+
Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector.,Huo Y, Zhao H, Dong Q, Jiang T Nat Microbiol. 2023 Mar;8(3):522-532. doi: 10.1038/s41564-022-01316-4. Epub 2023 , Jan 26. PMID:36702942<ref>PMID:36702942</ref>
-
Description: Cryo-EM structure of Cas7-11-crRNA bound to target RNA-2
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Qinghua, D]]
+
<div class="pdbe-citations 7ynb" style="background-color:#fffaf0;"></div>
-
[[Category: Tao, J]]
+
== References ==
-
[[Category: Hongshen, Z]]
+
<references/>
-
[[Category: Yangao, H]]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Desulfonema ishimotonii]]
 +
[[Category: Large Structures]]
 +
[[Category: Dong Q]]
 +
[[Category: Huo Y]]
 +
[[Category: Jiang T]]
 +
[[Category: Zhao H]]

Current revision

Cryo-EM structure of Cas7-11-crRNA bound to target RNA-2

PDB ID 7ynb

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7ynb"
Personal tools