8dyo

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'''Unreleased structure'''
 
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The entry 8dyo is ON HOLD
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==Cryo-EM structure of Importin-4 bound to RanGTP==
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<StructureSection load='8dyo' size='340' side='right'caption='[[8dyo]], [[Resolution|resolution]] 7.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8dyo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DYO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dyo OCA], [https://pdbe.org/8dyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dyo RCSB], [https://www.ebi.ac.uk/pdbsum/8dyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dyo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IPO4_HUMAN IPO4_HUMAN] Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.<ref>PMID:11823430</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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IMPORTIN-4, the primary nuclear import receptor of core histones H3 and H4, binds the H3-H4 dimer and histone chaperone ASF1 prior to nuclear import. However, how H3-H3-ASF1 is recognized for transport cannot be explained by available crystal structures of IMPORTIN-4-histone tail peptide complexes. Our 3.5-A IMPORTIN-4-H3-H4-ASF1 cryoelectron microscopy structure reveals the full nuclear import complex and shows a binding mode different from suggested by previous structures. The N-terminal half of IMPORTIN-4 clamps the globular H3-H4 domain and H3 alphaN helix, while its C-terminal half binds the H3 N-terminal tail weakly; tail contribution to binding energy is negligible. ASF1 binds H3-H4 without contacting IMPORTIN-4. Together, ASF1 and IMPORTIN-4 shield nucleosomal H3-H4 surfaces to chaperone and import it into the nucleus where RanGTP binds IMPORTIN-4, causing large conformational changes to release H3-H4-ASF1. This work explains how full-length H3-H4 binds IMPORTIN-4 in the cytoplasm and how it is released in the nucleus.
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Authors: Bernardes, N.E., Fung, H.Y.J., Li, Y., Chen, Z., Chook, Y.M.
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Structure of IMPORTIN-4 bound to the H3-H4-ASF1 histone-histone chaperone complex.,Bernardes NE, Fung HYJ, Li Y, Chen Z, Chook YM Proc Natl Acad Sci U S A. 2022 Sep 20;119(38):e2207177119. doi: , 10.1073/pnas.2207177119. Epub 2022 Sep 14. PMID:36103578<ref>PMID:36103578</ref>
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Description: Cryo-EM structure of Importin-4 bound to RanGTP
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Bernardes, N.E]]
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<div class="pdbe-citations 8dyo" style="background-color:#fffaf0;"></div>
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[[Category: Chen, Z]]
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[[Category: Li, Y]]
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==See Also==
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[[Category: Fung, H.Y.J]]
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*[[Importin 3D structures|Importin 3D structures]]
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[[Category: Chook, Y.M]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Bernardes NE]]
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[[Category: Chen Z]]
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[[Category: Chook YM]]
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[[Category: Fung HYJ]]
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[[Category: Li Y]]

Current revision

Cryo-EM structure of Importin-4 bound to RanGTP

PDB ID 8dyo

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