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1hwq

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[[Image:1hwq.gif|left|200px]]
 
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==SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP==
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The line below this paragraph, containing "STRUCTURE_1hwq", creates the "Structure Box" on the page.
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<StructureSection load='1hwq' size='340' side='right'caption='[[1hwq]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hwq]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HWQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwq OCA], [https://pdbe.org/1hwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hwq RCSB], [https://www.ebi.ac.uk/pdbsum/1hwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwq ProSAT]</span></td></tr>
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{{STRUCTURE_1hwq| PDB=1hwq | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.
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'''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP'''
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A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA.,Flinders J, Dieckmann T J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168<ref>PMID:11350168</ref>
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==Overview==
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The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA].
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</div>
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<div class="pdbe-citations 1hwq" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11350168 11350168]
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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[[Category: Dieckmann, T.]]
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== References ==
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[[Category: Flinders, J C.]]
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<references/>
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[[Category: A+c base pair]]
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__TOC__
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[[Category: Protonated adenine]]
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</StructureSection>
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[[Category: Stem-loop]]
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[[Category: Large Structures]]
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[[Category: Tandem ga]]
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[[Category: Dieckmann T]]
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[[Category: Vs ribozyme]]
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[[Category: Flinders JC]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:18:14 2008''
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Current revision

SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP

PDB ID 1hwq

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