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| | <StructureSection load='3wu0' size='340' side='right'caption='[[3wu0]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='3wu0' size='340' side='right'caption='[[3wu0]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wu0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WU0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wu0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WU0 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wts|3wts]], [[3wtt|3wtt]], [[3wtu|3wtu]], [[3wtv|3wtv]], [[3wtw|3wtw]], [[3wtx|3wtx]], [[3wty|3wty]], [[3wtz|3wtz]], [[3wu1|3wu1]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ETS1, EWSR2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wu0 OCA], [https://pdbe.org/3wu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wu0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wu0 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wu0 OCA], [https://pdbe.org/3wu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wu0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wu0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ETS1_HUMAN ETS1_HUMAN]] Transcription factor.<ref>PMID:10698492</ref>
| + | [https://www.uniprot.org/uniprot/ETS1_HUMAN ETS1_HUMAN] Transcription factor.<ref>PMID:10698492</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hamada, K]] | + | [[Category: Hamada K]] |
| - | [[Category: Ogata, K]] | + | [[Category: Ogata K]] |
| - | [[Category: Shiina, M]] | + | [[Category: Shiina M]] |
| - | [[Category: Autoinhibition]]
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| - | [[Category: Dna-binding]]
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| - | [[Category: Ets domain]]
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| - | [[Category: Ets-1]]
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| - | [[Category: Isopeptide bond]]
| + | |
| - | [[Category: Nucleus]]
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| - | [[Category: Phosphoprotein]]
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| - | [[Category: Phosphorylation]]
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| - | [[Category: Proto-oncogene]]
| + | |
| - | [[Category: Transcription]]
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| - | [[Category: Transcription regulation]]
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| Structural highlights
Function
ETS1_HUMAN Transcription factor.[1]
Publication Abstract from PubMed
Cooperative assemblies of transcription factors (TFs) on target gene enhancers coordinate cell proliferation, fate specification, and differentiation through precise and complicated transcriptional mechanisms. Chemical modifications, such as phosphorylation, of TFs induced by cell signaling, further modulate the dynamic cooperativity of TFs. In this study, we found that various Ets1-containing TF-DNA complexes respond differently to calcium-induced phosphorylation of Ets1, which is known to inhibit Ets1-DNA binding. Crystallographic analysis of a complex comprising Ets1, Runx1, and CBFbeta at the TCRalpha enhancer, revealed that Ets1 acquires robust binding stability in the Runx1 and DNA-complexed state, via allosteric mechanisms. This allows phosphorylated Ets1 to be retained at the TCRalpha enhancer with Runx1, in contrast to other Ets1 target gene enhancers including mb-1 and stromelysin-1. This study provides a structure-based model for cell signaling-dependent regulation of target genes, mediated via chemical modification of TFs.
A novel allosteric mechanism on protein-DNA interactions underlying the phosphorylation-dependent regulation of Ets1 target gene expressions.,Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Yamamoto M, Ogata K J Mol Biol. 2014 Jul 29. pii: S0022-2836(14)00368-4. doi:, 10.1016/j.jmb.2014.07.020. PMID:25083921[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li R, Pei H, Watson DK, Papas TS. EAP1/Daxx interacts with ETS1 and represses transcriptional activation of ETS1 target genes. Oncogene. 2000 Feb 10;19(6):745-53. PMID:10698492 doi:10.1038/sj.onc.1203385
- ↑ Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Yamamoto M, Ogata K. A novel allosteric mechanism on protein-DNA interactions underlying the phosphorylation-dependent regulation of Ets1 target gene expressions. J Mol Biol. 2014 Jul 29. pii: S0022-2836(14)00368-4. doi:, 10.1016/j.jmb.2014.07.020. PMID:25083921 doi:http://dx.doi.org/10.1016/j.jmb.2014.07.020
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