1g25

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(New page: 200px<br /> <applet load="1g25" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g25" /> '''SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN...)
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[[Image:1g25.gif|left|200px]]<br />
 
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<applet load="1g25" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g25" />
 
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'''SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HUMAN TFIIH MAT1 SUBUNIT'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HUMAN TFIIH MAT1 SUBUNIT==
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The human MAT1 protein belongs to the cyclin-dependent kinase-activating, kinase complex, which is functionally associated to the transcription/DNA, repair factor TFIIH. The N-terminal region of MAT1 consists of a C3HC4, RING finger, which contributes to optimal TFIIH transcriptional, activities. We report here the solution structure of the human MAT1 RING, finger domain (Met(1)-Asp(65)) as determined by (1)H NMR spectroscopy. The, MAT1 RING finger domain presents the expected betaalphabetabeta topology, with two interleaved zinc-binding sites conserved among the RING family., However, the presence of an additional helical segment in the N-terminal, part of the domain and a conserved hydrophobic central beta strand are the, defining features of this new structure and more generally of the MAT1, RING finger subfamily. Comparison of electrostatic surfaces of RING finger, structures shows that the RING finger domain of MAT1 presents a remarkable, positively charged surface. The functional implications of these MAT1 RING, finger features are discussed.
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<StructureSection load='1g25' size='340' side='right'caption='[[1g25]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G25 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g25 OCA], [https://pdbe.org/1g25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g25 RCSB], [https://www.ebi.ac.uk/pdbsum/1g25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g25 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MAT1_HUMAN MAT1_HUMAN] Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.<ref>PMID:10024882</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g2/1g25_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g25 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human MAT1 protein belongs to the cyclin-dependent kinase-activating kinase complex, which is functionally associated to the transcription/DNA repair factor TFIIH. The N-terminal region of MAT1 consists of a C3HC4 RING finger, which contributes to optimal TFIIH transcriptional activities. We report here the solution structure of the human MAT1 RING finger domain (Met(1)-Asp(65)) as determined by (1)H NMR spectroscopy. The MAT1 RING finger domain presents the expected betaalphabetabeta topology with two interleaved zinc-binding sites conserved among the RING family. However, the presence of an additional helical segment in the N-terminal part of the domain and a conserved hydrophobic central beta strand are the defining features of this new structure and more generally of the MAT1 RING finger subfamily. Comparison of electrostatic surfaces of RING finger structures shows that the RING finger domain of MAT1 presents a remarkable positively charged surface. The functional implications of these MAT1 RING finger features are discussed.
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==Disease==
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Solution structure of the N-terminal domain of the human TFIIH MAT1 subunit: new insights into the RING finger family.,Gervais V, Busso D, Wasielewski E, Poterszman A, Egly JM, Thierry JC, Kieffer B J Biol Chem. 2001 Mar 9;276(10):7457-64. Epub 2000 Oct 30. PMID:11056162<ref>PMID:11056162</ref>
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Known diseases associated with this structure: Hypermethioninemia, persistent, autosomal dominant, due to methionine adenosyltransferase I/III deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=250850 250850]], Methionine adenosyltransferase deficiency, autosomal recessive OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=250850 250850]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1G25 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G25 OCA].
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</div>
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<div class="pdbe-citations 1g25" style="background-color:#fffaf0;"></div>
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==Reference==
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== References ==
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Solution structure of the N-terminal domain of the human TFIIH MAT1 subunit: new insights into the RING finger family., Gervais V, Busso D, Wasielewski E, Poterszman A, Egly JM, Thierry JC, Kieffer B, J Biol Chem. 2001 Mar 9;276(10):7457-64. Epub 2000 Oct 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11056162 11056162]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Busso, D.]]
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[[Category: Busso D]]
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[[Category: Egly, J.M.]]
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[[Category: Egly JM]]
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[[Category: Gervais, V.]]
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[[Category: Gervais V]]
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[[Category: Kieffer, B.]]
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[[Category: Kieffer B]]
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[[Category: Poterszman, A.]]
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[[Category: Poterszman A]]
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[[Category: Thierry, J.C.]]
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[[Category: Thierry JC]]
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[[Category: Wasielewski, E.]]
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[[Category: Wasielewski E]]
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[[Category: ZN]]
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[[Category: ring finger (c3hc4)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:59:41 2007''
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Current revision

SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HUMAN TFIIH MAT1 SUBUNIT

PDB ID 1g25

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