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7yg8

From Proteopedia

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Current revision (06:44, 29 March 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 7yg8 is ON HOLD until Paper Publication
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==Cryo-EM structure of Tetrahymena ribozyme conformation 5 undergoing the second-step self-splicing==
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<StructureSection load='7yg8' size='340' side='right'caption='[[7yg8]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7yg8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena Tetrahymena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YG8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yg8 OCA], [https://pdbe.org/7yg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yg8 RCSB], [https://www.ebi.ac.uk/pdbsum/7yg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yg8 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing.
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Authors:
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Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.,Li S, Palo MZ, Zhang X, Pintilie G, Zhang K Nat Commun. 2023 Mar 16;14(1):1294. doi: 10.1038/s41467-023-36724-5. PMID:36928031<ref>PMID:36928031</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 7yg8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Tetrahymena]]
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[[Category: Greg P]]
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[[Category: Li S]]
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[[Category: Michael ZP]]
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[[Category: Zhang K]]
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[[Category: Zhang X]]

Current revision

Cryo-EM structure of Tetrahymena ribozyme conformation 5 undergoing the second-step self-splicing

PDB ID 7yg8

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