8dq0
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Quorum-sensing receptor RhlR bound to PqsE== | |
| + | <StructureSection load='8dq0' size='340' side='right'caption='[[8dq0]], [[Resolution|resolution]] 3.74Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8dq0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DQ0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.74Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K5G:4-(3-bromophenoxy)-N-[(3S)-2-oxothiolan-3-yl]butanamide'>K5G</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dq0 OCA], [https://pdbe.org/8dq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dq0 RCSB], [https://www.ebi.ac.uk/pdbsum/8dq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dq0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RHLR_PSEAE RHLR_PSEAE] Quorum-sensing regulator that controls the expression of multiple virulence factors in response to extracellular signaling molecules called autoinducers (PubMed:15576196, PubMed:7494482, PubMed:8522523). Involved, among others, in the transcriptional regulation of genes that are responsible for rhamnolipid surfactant biosynthesis (PubMed:14526008, PubMed:7604006, PubMed:8144472). Acts by binding to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer (PubMed:14526008). In the former case it activates transcription of the promoter, whereas in the latter it acts as a transcriptional repressor (PubMed:14526008). Also regulates the expression of the rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, by binding to the rml box in the promoter region (PubMed:22262098). In addition, is involved in the regulation of the production of elastase (lasB) and pyocyanine (PubMed:7604006, PubMed:8144472, PubMed:8522523).<ref>PMID:14526008</ref> <ref>PMID:15576196</ref> <ref>PMID:22262098</ref> <ref>PMID:7494482</ref> <ref>PMID:7604006</ref> <ref>PMID:8144472</ref> <ref>PMID:8522523</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pseudomonas aeruginosa is an opportunistic pathogen that is responsible for thousands of deaths every year in the United States. P. aeruginosa virulence factor production is mediated by quorum sensing, a mechanism of bacterial cell-cell communication that relies on the production and detection of signal molecules called autoinducers. In P. aeruginosa, the transcription factor receptor RhlR is activated by a RhlI-synthesized autoinducer. We recently showed that RhlR-dependent transcription is enhanced by a physical interaction with the enzyme PqsE via increased affinity of RhlR for promoter DNA. However, the molecular basis for complex formation and how complex formation enhanced RhlR transcriptional activity remained unclear. Here, we report the structure of ligand-bound RhlR in complex with PqsE. Additionally, we determined the structure of the complex bound with DNA, revealing the mechanism by which RhlR-mediated transcription is enhanced by PqsE, thereby establishing the molecular basis for RhlR-dependent virulence factor production in P. aeruginosa. | ||
| - | + | Structure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum-sensing gene regulation.,Feathers JR, Richael EK, Simanek KA, Fromme JC, Paczkowski JE Structure. 2022 Dec 1;30(12):1626-1636.e4. doi: 10.1016/j.str.2022.10.008. Epub , 2022 Nov 14. PMID:36379213<ref>PMID:36379213</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8dq0" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Thioesterase 3D structures|Thioesterase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa]] | ||
| + | [[Category: Feathers JR]] | ||
| + | [[Category: Fromme JC]] | ||
| + | [[Category: Paczkowski JE]] | ||
Current revision
Quorum-sensing receptor RhlR bound to PqsE
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