8e3q

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(New page: '''Unreleased structure''' The entry 8e3q is ON HOLD Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L. Description: CryoEM structure of mPSF in complex with the non-canonical PAS AA...)
Current revision (05:24, 12 June 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8e3q is ON HOLD
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==CRYO-EM STRUCTURE OF the human MPSF==
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<StructureSection load='8e3q' size='340' side='right'caption='[[8e3q]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8e3q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E3Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E3Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e3q OCA], [https://pdbe.org/8e3q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e3q RCSB], [https://www.ebi.ac.uk/pdbsum/8e3q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e3q ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The polyadenylation signal (PAS) is a key sequence element for 3'-end cleavage and polyadenylation of messenger RNA precursors (pre-mRNAs). This hexanucleotide motif is recognized by the mammalian polyadenylation specificity factor (mPSF), consisting of CPSF160, WDR33, CPSF30, and Fip1 subunits. Recent studies have revealed how the AAUAAA PAS, the most frequently observed PAS, is recognized by mPSF. We report here the structure of human mPSF in complex with the AUUAAA PAS, the second most frequently identified PAS. Conformational differences are observed for the A1 and U2 nucleotides in AUUAAA compared to the A1 and A2 nucleotides in AAUAAA, while the binding modes of the remaining 4 nt are essentially identical. The 5' phosphate of U2 moves by 2.6 A and the U2 base is placed near the six-membered ring of A2 in AAUAAA, where it makes two hydrogen bonds with zinc finger 2 (ZF2) of CPSF30, which undergoes conformational changes as well. We also attempted to determine the binding modes of two rare PAS hexamers, AAGAAA and GAUAAA, but did not observe the RNA in the cryo-electron microscopy density. The residues in CPSF30 (ZF2 and ZF3) and WDR33 that recognize PAS are disordered in these two structures.
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Authors: Gutierrez, P.A., Wei, J., Sun, Y., Tong, L.
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Molecular basis for the recognition of the AUUAAA polyadenylation signal by mPSF.,Gutierrez PA, Wei J, Sun Y, Tong L RNA. 2022 Nov;28(11):1534-1541. doi: 10.1261/rna.079322.122. Epub 2022 Sep 6. PMID:36130077<ref>PMID:36130077</ref>
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Description: CryoEM structure of mPSF in complex with the non-canonical PAS AAGAAA (Not observed)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Tong, L]]
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<div class="pdbe-citations 8e3q" style="background-color:#fffaf0;"></div>
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[[Category: Gutierrez, P.A]]
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== References ==
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[[Category: Wei, J]]
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<references/>
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[[Category: Sun, Y]]
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Gutierrez PA]]
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[[Category: Sun Y]]
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[[Category: Tong L]]
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[[Category: Wei J]]

Current revision

CRYO-EM STRUCTURE OF the human MPSF

PDB ID 8e3q

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