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| | ==Solution Structure of the UBA Domain of Human NBR1== | | ==Solution Structure of the UBA Domain of Human NBR1== |
| - | <StructureSection load='2mgw' size='340' side='right'caption='[[2mgw]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mgw' size='340' side='right'caption='[[2mgw]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mgw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MGW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mgw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MGW FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2mj5|2mj5]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mgw OCA], [https://pdbe.org/2mgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mgw RCSB], [https://www.ebi.ac.uk/pdbsum/2mgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mgw ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mgw OCA], [https://pdbe.org/2mgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mgw RCSB], [https://www.ebi.ac.uk/pdbsum/2mgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mgw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/NBR1_HUMAN NBR1_HUMAN]] Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.<ref>PMID:19250911</ref>
| + | [https://www.uniprot.org/uniprot/NBR1_HUMAN NBR1_HUMAN] Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.<ref>PMID:19250911</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Komatsu, M]] | + | [[Category: Komatsu M]] |
| - | [[Category: Morimoto, D]] | + | [[Category: Morimoto D]] |
| - | [[Category: Shirakawa, M]] | + | [[Category: Shirakawa M]] |
| - | [[Category: Sugase, K]] | + | [[Category: Sugase K]] |
| - | [[Category: Tochio, H]] | + | [[Category: Tochio H]] |
| - | [[Category: Walinda, E]] | + | [[Category: Walinda E]] |
| - | [[Category: Autophagy]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Protein degradation]]
| + | |
| - | [[Category: Ubiquitin binding]]
| + | |
| Structural highlights
Function
NBR1_HUMAN Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.[1]
Publication Abstract from PubMed
NBR1 (neighbor of BRCA1 gene 1) is a protein commonly found in ubiquitin-positive inclusions in neurodegenerative diseases. Due to its high architectural similarity to the well-studied autophagy receptor protein p62/SQSTM1, NBR1 has been thought to analogously bind to ubiquitin-marked autophagic substrates via its carboxy-terminal ubiquitin-associated (UBA) domain and deliver them to autophagosomes for degradation. Unexpectedly, we find that NBR1 differs from p62 in its UBA structure and accordingly in its interaction with ubiquitin. Structural differences are observed on helix alpha-3, which is tilted further from helix alpha-2 and extended by approximately one turn in NBR1. This results not only in inhibition of a p62-type self-dimerization of NBR1 UBA, but also in a significantly higher affinity for monoubiquitin as compared with p62 UBA. Importantly, the NBR1 UBA-ubiquitin complex structure shows that the negative charge of the side chain in front of the conserved MGF motif in the UBA plays an integral role in the recognition of ubiquitin. In addition, NMR and ITC experiments show that NBR1 UBA binds to each monomeric unit of polyubiquitin with similar affinity and by the same surface used for binding to monoubiquitin. This indicates that NBR1 lacks polyubiquitin linkage-type specificity, in good agreement with the non-specific linkages observed in intracellular ubiquitin-positive inclusions. Consequently, our results demonstrate that the structural differences between NBR1 UBA and p62 UBA result in a much higher affinity of NBR1 for ubiquitin, which in turn suggests that NBR1 may form intracellular inclusions with ubiquitylated autophagic substrates more efficiently than p62.
Solution Structure of the Ubiquitin-associated (UBA) Domain of Human Autophagy Receptor NBR1 and its Interaction with Ubiquitin and Polyubiquitin.,Walinda E, Morimoto D, Sugase K, Konuma T, Tochio H, Shirakawa M J Biol Chem. 2014 Apr 1. PMID:24692539[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kirkin V, Lamark T, Sou YS, Bjorkoy G, Nunn JL, Bruun JA, Shvets E, McEwan DG, Clausen TH, Wild P, Bilusic I, Theurillat JP, Overvatn A, Ishii T, Elazar Z, Komatsu M, Dikic I, Johansen T. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol Cell. 2009 Feb 27;33(4):505-16. doi: 10.1016/j.molcel.2009.01.020. PMID:19250911 doi:10.1016/j.molcel.2009.01.020
- ↑ Walinda E, Morimoto D, Sugase K, Konuma T, Tochio H, Shirakawa M. Solution Structure of the Ubiquitin-associated (UBA) Domain of Human Autophagy Receptor NBR1 and its Interaction with Ubiquitin and Polyubiquitin. J Biol Chem. 2014 Apr 1. PMID:24692539 doi:http://dx.doi.org/10.1074/jbc.M114.555441
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