3wg6

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of conjugated polyketone reductase C1 from Candida parapsilosis complexed with NADPH

Structural highlights

3wg6 is a 4 chain structure with sequence from Candida parapsilosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CPRC1_CANPA NADPH-dependent conjugated polyketone reductase with broad substrate specificity and strict stereospecificity. Reduces ketopantoyl lactone (2-dehydropantolactone) and isatin. Does not act on menadione, p-nitrobenzaldehyde and pyridine-3-aldehyde.^[1] ^[2]

Publication Abstract from PubMed

Conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 is a member of the aldo-keto reductase (AKR) superfamily and reduces ketopantoyl lactone to D-pantoyl lactone in a NADPH-dependent and stereospecific manner. We determined the crystal structure of CPR-C1.NADPH complex at 2.20 A resolution. CPR-C1 adopted a triose-phosphate isomerase (TIM) barrel fold at the core of the structure in which Thr25 and Lys26 of the GXGTX motif bind uniquely to the adenosine 2'-phosphate group of NADPH. This finding provides a novel structural basis for NADPH binding of the AKR superfamily. (c) Proteins 2013;. (c) 2013 Wiley Periodicals, Inc.

Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH.,Qin HM, Yamamura A, Miyakawa T, Kataoka M, Maruoka S, Ohtsuka J, Nagata K, Shimizu S, Tanokura M Proteins. 2013 Jul 15. doi: 10.1002/prot.24363. PMID:23852710^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hidalgo AR, Akond MA, Kita K, Kataoka M, Shimizu S. Isolation and primary structural analysis of two conjugated polyketone reductases from Candida parapsilosis. Biosci Biotechnol Biochem. 2001 Dec;65(12):2785-8. doi: 10.1271/bbb.65.2785. PMID:11826979 doi:http://dx.doi.org/10.1271/bbb.65.2785
↑ Kataoka M, Delacruz-Hidalgo AR, Akond MA, Sakuradani E, Kita K, Shimizu S. Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis. Appl Microbiol Biotechnol. 2004 Apr;64(3):359-66. doi: 10.1007/s00253-003-1484-3., Epub 2003 Oct 31. PMID:14593510 doi:http://dx.doi.org/10.1007/s00253-003-1484-3
↑ Qin HM, Yamamura A, Miyakawa T, Kataoka M, Maruoka S, Ohtsuka J, Nagata K, Shimizu S, Tanokura M. Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH. Proteins. 2013 Jul 15. doi: 10.1002/prot.24363. PMID:23852710 doi:10.1002/prot.24363

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3wg6"

@@ Line 3: / Line 3: @@
 <StructureSection load='3wg6' size='340' side='right'caption='[[3wg6]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wg6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WG6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wg6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WG6 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wg6 OCA], [https://pdbe.org/3wg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wg6 RCSB], [https://www.ebi.ac.uk/pdbsum/3wg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wg6 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CPRC1_CANPA CPRC1_CANPA] NADPH-dependent conjugated polyketone reductase with broad substrate specificity and strict stereospecificity. Reduces ketopantoyl lactone (2-dehydropantolactone) and isatin. Does not act on menadione, p-nitrobenzaldehyde and pyridine-3-aldehyde.<ref>PMID:11826979</ref> <ref>PMID:14593510</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Candida parapsilosis]]
 [[Category: Large Structures]]
-[[Category: Kataoka, M]]
+[[Category: Kataoka M]]
-[[Category: Maruoka, S]]
+[[Category: Maruoka S]]
-[[Category: Miyakawa, T]]
+[[Category: Miyakawa T]]
-[[Category: Nagata, K]]
+[[Category: Nagata K]]
-[[Category: Ohtsuka, J]]
+[[Category: Ohtsuka J]]
-[[Category: Qin, H M]]
+[[Category: Qin H-M]]
-[[Category: Shimizu, S]]
+[[Category: Shimizu S]]
-[[Category: Tanokura, M]]
+[[Category: Tanokura M]]
-[[Category: Yamamura, A]]
+[[Category: Yamamura A]]
-[[Category: Akr superfamily]]
-[[Category: D-pantoyl lactone]]
-[[Category: Oxidoreductase]]
-[[Category: Tim barrel]]

3wg6

From Proteopedia

Current revision

Crystal structure of conjugated polyketone reductase C1 from Candida parapsilosis complexed with NADPH

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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