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4ilu

From Proteopedia

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Crystal structure of Mycobacterium tuberculosis CarD

Structural highlights

4ilu is a 1 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CARD_MYCTU Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.^[1] ^[2]

Publication Abstract from PubMed

Mycobacterium tuberculosis (Mtb) CarD is an essential transcriptional regulator that binds RNA polymerase and plays an important role in reprogramming transcription machinery under diverse stress conditions. Here, we report the crystal structure of CarD at 2.3 A resolution, that represents the first structural description of CarD/CdnL-Like family of proteins. CarD adopts an overall bi-lobed structural architecture where N-terminal domain resembles 'tudor-like' domain and C-terminal domain adopts a novel five helical fold that lacks the predicted leucine zipper structural motif. The structure reveals dimeric state of CarD resulting from beta-strand swapping between the N-terminal domains of each individual subunits. The structure provides crucial insights into the possible mode(s) of CarD/RNAP interactions. Proteins 2013; (c) 2013 Wiley Periodicals, Inc.

Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture.,Kaur G, Dutta D, Thakur KG Proteins. 2013 Sep 30. doi: 10.1002/prot.24419. PMID:24115125^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Stallings CL, Stephanou NC, Chu L, Hochschild A, Nickels BE, Glickman MS. CarD is an essential regulator of rRNA transcription required for Mycobacterium tuberculosis persistence. Cell. 2009 Jul 10;138(1):146-59. doi: 10.1016/j.cell.2009.04.041. PMID:19596241 doi:10.1016/j.cell.2009.04.041
↑ Weiss LA, Harrison PG, Nickels BE, Glickman MS, Campbell EA, Darst SA, Stallings CL. Interaction of CarD with RNA polymerase mediates Mycobacterium tuberculosis viability, rifampin resistance, and pathogenesis. J Bacteriol. 2012 Oct;194(20):5621-31. Epub 2012 Aug 17. PMID:22904282 doi:10.1128/JB.00879-12
↑ Kaur G, Dutta D, Thakur KG. Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture. Proteins. 2013 Sep 30. doi: 10.1002/prot.24419. PMID:24115125 doi:http://dx.doi.org/10.1002/prot.24419

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ilu"

Categories: Large Structures | Mycobacterium tuberculosis | Kaur G | Thakur KG

@@ Line 3: / Line 3: @@
 <StructureSection load='4ilu' size='340' side='right'caption='[[4ilu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ilu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ILU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ilu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ILU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4mfr|4mfr]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ilu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilu OCA], [https://pdbe.org/4ilu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ilu RCSB], [https://www.ebi.ac.uk/pdbsum/4ilu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ilu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CARD_MYCTU CARD_MYCTU]] Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.<ref>PMID:19596241</ref> <ref>PMID:22904282</ref>
+[https://www.uniprot.org/uniprot/CARD_MYCTU CARD_MYCTU] Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.<ref>PMID:19596241</ref> <ref>PMID:22904282</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Kaur, G]]
+[[Category: Mycobacterium tuberculosis]]
-[[Category: Thakur, K G]]
+[[Category: Kaur G]]
-[[Category: Five helical fold]]
+[[Category: Thakur KG]]
-[[Category: Rna polymerase binding]]
-[[Category: Transcription]]
-[[Category: Transcription regulation]]
-[[Category: Tudor like domain]]

4ilu

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Current revision

Crystal structure of Mycobacterium tuberculosis CarD

Structural highlights

Function

Publication Abstract from PubMed

References

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