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4q65

From Proteopedia

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Structure of the E. coli Peptide Transporter YbgH

Structural highlights

4q65 is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DTPD_ECOLI Probable proton-dependent permease that transports dipeptides (By similarity).

Publication Abstract from PubMed

E. coli YbgH belongs to the family of proton-dependent oligopeptide transporters (POTs), a subfamily of the major facilitator superfamily (MFS) of secondary active transporters. Like other MFS transporters, POT proteins switch between two major conformations during substrate transport. Apart from possessing a canonical 12-helix, two-domain transmembrane (TM) core, prokaryotic POT proteins usually have two TM helices inserted between the two domains. Here we determined the crystal structure of YbgH in its inward-facing conformation. Our structure-based functional studies investigated the roles of both the POT signature motif 2 and the inserted interdomain TM helix pair in the stabilization and regulation of the major conformational change in MFS/POT transporters. Furthermore, of all the proton-titratable amino acid residues, Glu21 is the only conserved one (among POTs) located in the central cavity and is critical for in vivo transport. Together, our results support the notion that MFS symporters utilize a transport mechanism based on substrate-protonation coupling.

Crystal Structure of the E. coli Peptide Transporter YbgH.,Zhao Y, Mao G, Liu M, Zhang L, Wang X, Zhang XC Structure. 2014 Aug 5;22(8):1152-60. doi: 10.1016/j.str.2014.06.008. Epub 2014, Jul 24. PMID:25066136^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhao Y, Mao G, Liu M, Zhang L, Wang X, Zhang XC. Crystal Structure of the E. coli Peptide Transporter YbgH. Structure. 2014 Aug 5;22(8):1152-60. doi: 10.1016/j.str.2014.06.008. Epub 2014, Jul 24. PMID:25066136 doi:http://dx.doi.org/10.1016/j.str.2014.06.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4q65"

@@ Line 3: / Line 3: @@
 <StructureSection load='4q65' size='340' side='right'caption='[[4q65]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4q65]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q65 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4q65]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q65 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q65 OCA], [https://pdbe.org/4q65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q65 RCSB], [https://www.ebi.ac.uk/pdbsum/4q65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q65 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q65 OCA], [https://pdbe.org/4q65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q65 RCSB], [https://www.ebi.ac.uk/pdbsum/4q65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q65 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/DTPD_ECOLI DTPD_ECOLI]] Probable proton-dependent permease that transports dipeptides (By similarity).
+[https://www.uniprot.org/uniprot/DTPD_ECOLI DTPD_ECOLI] Probable proton-dependent permease that transports dipeptides (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Liu, M]]
+[[Category: Liu M]]
-[[Category: Mao, G]]
+[[Category: Mao G]]
-[[Category: Wang, X]]
+[[Category: Wang X]]
-[[Category: Zhang, C]]
+[[Category: Zhang C]]
-[[Category: Zhao, Y]]
+[[Category: Zhao Y]]
-[[Category: Mfs fold motif some]]
-[[Category: Peptide transporter]]
-[[Category: Transport protein]]

4q65

From Proteopedia

Current revision

Structure of the E. coli Peptide Transporter YbgH

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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