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8dss
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray crystal structure of Geobacillus stearothermophilus ComEA== | |
| + | <StructureSection load='8dss' size='340' side='right'caption='[[8dss]], [[Resolution|resolution]] 3.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8dss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus_ATCC_7953 Geobacillus stearothermophilus ATCC 7953]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DSS FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dss OCA], [https://pdbe.org/8dss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dss RCSB], [https://www.ebi.ac.uk/pdbsum/8dss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dss ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0K9HI54_GEOSE A0A0K9HI54_GEOSE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | An essential step in bacterial transformation is the uptake of DNA into the periplasm, across the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin peptidoglycan layer of Gram-negative bacteria. ComEA, a DNA-binding protein widely conserved in transformable bacteria, is required for this uptake step. Here we determine X-ray crystal structures of ComEA from two Gram-positive species, Bacillus subtilis and Geobacillus stearothermophilus, identifying a domain that is absent in Gram-negative bacteria. X-ray crystallographic, genetic, and analytical ultracentrifugation (AUC) analyses reveal that this domain drives ComEA oligomerization, which we show is required for transformation. We use multi-wavelength AUC (MW-AUC) to characterize the interaction between DNA and the ComEA DNA-binding domain. Finally, we present a model for the interaction of the ComEA DNA-binding domain with DNA, suggesting that ComEA oligomerization may provide a pulling force that drives DNA uptake across the thick cell walls of Gram-positive bacteria. | ||
| - | + | Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria.,Ahmed I, Hahn J, Henrickson A, Khaja FT, Demeler B, Dubnau D, Neiditch MB Nat Commun. 2022 Dec 13;13(1):7724. doi: 10.1038/s41467-022-35129-0. PMID:36513643<ref>PMID:36513643</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8dss" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Geobacillus stearothermophilus ATCC 7953]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ahmed I]] | ||
| + | [[Category: Neiditch MB]] | ||
Current revision
X-ray crystal structure of Geobacillus stearothermophilus ComEA
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