4b2g

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of an Indole-3-Acetic Acid Amido Synthase from Vitis vinifera Involved in Auxin Homeostasis

Structural highlights

4b2g is a 2 chain structure with sequence from Vitis vinifera. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

F6H697_VITVI

Publication Abstract from PubMed

Auxins are important for plant growth and development, including the control of fruit ripening. Conjugation to amino acids by indole-3-acetic acid (IAA)-amido synthetases is an important part of auxin homeostasis. The structure of the auxin-conjugating Gretchen Hagen3-1 (GH3-1) enzyme from grapevine (Vitis vinifera), in complex with an inhibitor (adenosine-5'-[2-(1H-indol-3-yl)ethyl]phosphate), is presented. Comparison with a previously published benzoate-conjugating enzyme from Arabidopsis thaliana indicates that grapevine GH3-1 has a highly similar domain structure and also undergoes a large conformational change during catalysis. Mutational analyses and structural comparisons with other proteins have identified residues likely to be involved in acyl group, amino acid, and ATP substrate binding. Vv GH3-1 is a monomer in solution and requires magnesium ions solely for the adenlyation reaction. Modeling of IAA and two synthetic auxins, benzothiazole-2-oxyacetic acid (BTOA) and 1-naphthaleneacetic acid (NAA), into the active site indicates that NAA and BTOA are likely to be poor substrates for this enzyme, confirming previous enzyme kinetic studies. This suggests a reason for the increased effectiveness of NAA and BTOA as auxins in planta and provides a tool for designing new and effective auxins.

Crystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin Homeostasis.,Peat TS, Bottcher C, Newman J, Lucent D, Cowieson N, Davies C Plant Cell. 2012 Nov 9. PMID:23136372^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Peat TS, Bottcher C, Newman J, Lucent D, Cowieson N, Davies C. Crystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin Homeostasis. Plant Cell. 2012 Nov 9. PMID:23136372 doi:http://dx.doi.org/10.1105/tpc.112.102921

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4b2g"

@@ Line 3: / Line 3: @@
 <StructureSection load='4b2g' size='340' side='right'caption='[[4b2g]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4b2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Grape Grape]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B2G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4b2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B2G FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=V1N:[(2S,3R,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]+2-(1H-INDOL-3-YL)ETHYL+HYDROGEN+PHOSPHATE'>V1N</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=V1N:[(2S,3R,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]+2-(1H-INDOL-3-YL)ETHYL+HYDROGEN+PHOSPHATE'>V1N</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b2g OCA], [https://pdbe.org/4b2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b2g RCSB], [https://www.ebi.ac.uk/pdbsum/4b2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b2g ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/F6H697_VITVI F6H697_VITVI]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Grape]]
 [[Category: Large Structures]]
-[[Category: Bottcher, C]]
+[[Category: Vitis vinifera]]
-[[Category: Cowieson, N]]
+[[Category: Bottcher C]]
-[[Category: Davies, C]]
+[[Category: Cowieson N]]
-[[Category: Lucent, D]]
+[[Category: Davies C]]
-[[Category: Newman, J]]
+[[Category: Lucent D]]
-[[Category: Peat, T S]]
+[[Category: Newman J]]
-[[Category: Adenylate]]
+[[Category: Peat TS]]
-[[Category: Amino acid conjugation]]
-[[Category: Ignaling protein]]
-[[Category: Plant growth]]
-[[Category: Signaling protein]]

4b2g

From Proteopedia

Current revision

Crystal Structure of an Indole-3-Acetic Acid Amido Synthase from Vitis vinifera Involved in Auxin Homeostasis

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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