4boc

From Proteopedia

(Difference between revisions)

Current revision

Structure of mitochondrial RNA polymerase elongation complex

Structural highlights

4boc is a 4 chain structure with sequence from Homo sapiens and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.65Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPOM_HUMAN DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.^[1]

Publication Abstract from PubMed

Here we report the crystal structure of the human mitochondrial RNA polymerase (mtRNAP) transcription elongation complex, determined at 2.65-A resolution. The structure reveals a 9-bp hybrid formed between the DNA template and the RNA transcript and one turn of DNA both upstream and downstream of the hybrid. Comparisons with the distantly related RNA polymerase (RNAP) from bacteriophage T7 indicates conserved mechanisms for substrate binding and nucleotide incorporation but also strong mechanistic differences. Whereas T7 RNAP refolds during the transition from initiation to elongation, mtRNAP adopts an intermediary conformation that is capable of elongation without refolding. The intercalating hairpin that melts DNA during T7 RNAP initiation separates RNA from DNA during mtRNAP elongation. Newly synthesized RNA exits toward the pentatricopeptide repeat (PPR) domain, a unique feature of mtRNAP with conserved RNA-recognition motifs.

Structure of human mitochondrial RNA polymerase elongation complex.,Schwinghammer K, Cheung AC, Morozov YI, Agaronyan K, Temiakov D, Cramer P Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2683. PMID:24096365^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Minczuk M, He J, Duch AM, Ettema TJ, Chlebowski A, Dzionek K, Nijtmans LG, Huynen MA, Holt IJ. TEFM (c17orf42) is necessary for transcription of human mtDNA. Nucleic Acids Res. 2011 May;39(10):4284-99. doi: 10.1093/nar/gkq1224. Epub 2011, Jan 28. PMID:21278163 doi:10.1093/nar/gkq1224
↑ Schwinghammer K, Cheung AC, Morozov YI, Agaronyan K, Temiakov D, Cramer P. Structure of human mitochondrial RNA polymerase elongation complex. Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2683. PMID:24096365 doi:http://dx.doi.org/10.1038/nsmb.2683

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4boc"

@@ Line 3: / Line 3: @@
 <StructureSection load='4boc' size='340' side='right'caption='[[4boc]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4boc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BOC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4boc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BOC FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4boc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4boc OCA], [https://pdbe.org/4boc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4boc RCSB], [https://www.ebi.ac.uk/pdbsum/4boc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4boc ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RPOM_HUMAN RPOM_HUMAN]] DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.<ref>PMID:21278163</ref>
+[https://www.uniprot.org/uniprot/RPOM_HUMAN RPOM_HUMAN] DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.<ref>PMID:21278163</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: DNA-directed RNA polymerase]]
+[[Category: Homo sapiens]]
-[[Category: Human]]
 [[Category: Large Structures]]
-[[Category: Agaronyan, K]]
+[[Category: Synthetic construct]]
-[[Category: Cheung, A]]
+[[Category: Agaronyan K]]
-[[Category: Cramer, P]]
+[[Category: Cheung A]]
-[[Category: Morozov, Y]]
+[[Category: Cramer P]]
-[[Category: Schwinghammer, K]]
+[[Category: Morozov Y]]
-[[Category: Temiakov, D]]
+[[Category: Schwinghammer K]]
-[[Category: Dna-rna hybrid]]
+[[Category: Temiakov D]]
-[[Category: Mitochondria]]
-[[Category: Rna polymerase]]
-[[Category: Transcription]]
-[[Category: Transferase]]

4boc

From Proteopedia

Current revision

Structure of mitochondrial RNA polymerase elongation complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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