1i3h

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[[Image:1i3h.gif|left|200px]]
 
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==CONCANAVALIN A-DIMANNOSE STRUCTURE==
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The line below this paragraph, containing "STRUCTURE_1i3h", creates the "Structure Box" on the page.
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<StructureSection load='1i3h' size='340' side='right'caption='[[1i3h]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1i3h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I3H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900111:2alpha-alpha-mannobiose'>PRD_900111</scene></td></tr>
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{{STRUCTURE_1i3h| PDB=1i3h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i3h OCA], [https://pdbe.org/1i3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i3h RCSB], [https://www.ebi.ac.uk/pdbsum/1i3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i3h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CONA_CANEN CONA_CANEN] D-mannose specific lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i3/1i3h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i3h ConSurf].
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<div style="clear:both"></div>
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'''CONCANAVALIN A-DIMANNOSE STRUCTURE'''
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==See Also==
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*[[Concanavalin 3D structures|Concanavalin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The complex between concanavalin A (Con A) and alpha1-2 mannobiose (mannose alpha1-2 mannose) has been refined to 1.2 A resolution. This is the highest resolution structure reported for any sugar-lectin complex. As the native structure of Con A to 0.94 A resolution is already in the database, this gives us a unique opportunity to examine sugar-protein binding at high resolution. These data have allowed us to model a number of hydrogen atoms involved in the binding of the sugar to Con A, using the difference density map to place the hydrogen atoms. This map reveals the presence of the protonated form of Asp208 involved in binding. Asp208 is not protonated in the 0.94 A native structure. Our results clearly show that this residue is protonated and hydrogen bonds to the sugar. The structure accounts for the higher affinity of the alpha1-2 linked sugar when compared to other disaccharides. This structure identifies different interactions to those predicted by previous modelling studies. We believe that the additional data presented here will enable significant improvements to be made to the sugar-protein modelling algorithms.
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==About this Structure==
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1I3H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3H OCA].
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==Reference==
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The 1.2 A resolution structure of the Con A-dimannose complex., Sanders DA, Moothoo DN, Raftery J, Howard AJ, Helliwell JR, Naismith JH, J Mol Biol. 2001 Jul 20;310(4):875-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11453694 11453694]
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[[Category: Canavalia ensiformis]]
[[Category: Canavalia ensiformis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Helliwell, J R.]]
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[[Category: Helliwell JR]]
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[[Category: Howard, A J.]]
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[[Category: Howard AJ]]
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[[Category: Moothoo, D N.]]
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[[Category: Moothoo DN]]
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[[Category: Naismith, J H.]]
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[[Category: Naismith JH]]
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[[Category: Raftery, J.]]
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[[Category: Raftery J]]
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[[Category: Sanders, D A.R.]]
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[[Category: Sanders DAR]]
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[[Category: Concanavalin some]]
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[[Category: Protein-sugar complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:31:41 2008''
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Current revision

CONCANAVALIN A-DIMANNOSE STRUCTURE

PDB ID 1i3h

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