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4s0g
From Proteopedia
(Difference between revisions)
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==Crystal structure of PTPN3 (PTPH1) in complex with Eps15 pTyr849 P850V peptide== | ==Crystal structure of PTPN3 (PTPH1) in complex with Eps15 pTyr849 P850V peptide== | ||
| - | <StructureSection load='4s0g' size='340' side='right'caption='[[4s0g]]' scene=''> | + | <StructureSection load='4s0g' size='340' side='right'caption='[[4s0g]], [[Resolution|resolution]] 1.72Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S0G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4s0g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S0G FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s0g OCA], [https://pdbe.org/4s0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s0g RCSB], [https://www.ebi.ac.uk/pdbsum/4s0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s0g ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.723Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s0g OCA], [https://pdbe.org/4s0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s0g RCSB], [https://www.ebi.ac.uk/pdbsum/4s0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s0g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PTN3_HUMAN PTN3_HUMAN] May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Epidermal growth factor receptor (EGFR) pathway substrate 15 (Eps15) is a newly identified substrate for protein tyrosine phosphatase N3 (PTPN3), which belongs to the FERM-containing PTP subfamily comprising five members including PTPN3, N4, N13, N14, and N21. We solved the crystal structures of the PTPN3-Eps15 phosphopeptide complex and found that His812 of PTPN3 and Pro850 of Eps15 are responsible for the specific interaction between them. We defined the critical role of the additional residue Tyr676 of PTPN3, which is replaced by Ile939 in PTPN14, in recognition of tyrosine phosphorylated Eps15. The WPD loop necessary for catalysis is present in all members but not PTPN21. We identified that Glu instead of Asp in the WPE loop contributes to the catalytic incapability of PTPN21 due to an extended distance beyond protonation targeting a phosphotyrosine substrate. Together with in vivo validations, our results provide novel insights into the substrate specificity and plasticity of FERM-containing PTPs. | ||
| + | |||
| + | Substrate Specificity and Plasticity of FERM-Containing Protein Tyrosine Phosphatases.,Chen KE, Li MY, Chou CC, Ho MR, Chen GC, Meng TC, Wang AH Structure. 2015 Feb 18. pii: S0969-2126(15)00041-6. doi:, 10.1016/j.str.2015.01.017. PMID:25728925<ref>PMID:25728925</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4s0g" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]] | *[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chen K-E]] | [[Category: Chen K-E]] | ||
[[Category: Meng TC]] | [[Category: Meng TC]] | ||
[[Category: Wang AH-J]] | [[Category: Wang AH-J]] | ||
Current revision
Crystal structure of PTPN3 (PTPH1) in complex with Eps15 pTyr849 P850V peptide
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