8axn
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8axn is ON HOLD Authors: Lunelli, M. Description: Inner membrane ring and secretin N0 N1 domains of the type 3 secretion system of Shigella flexner...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Inner membrane ring and secretin N0 N1 domains of the type 3 secretion system of Shigella flexneri== | |
| + | <StructureSection load='8axn' size='340' side='right'caption='[[8axn]], [[Resolution|resolution]] 3.34Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8axn]] is a 64 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AXN FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8axn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8axn OCA], [https://pdbe.org/8axn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8axn RCSB], [https://www.ebi.ac.uk/pdbsum/8axn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8axn ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MXIG_SHIFL MXIG_SHIFL] Involved in the secretion of the Ipa antigens. Involved in the intracellular dissemination of Shigella. Part of the Mxi-Spa secretion apparatus. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The type III secretion system (T3SS) is a large, transmembrane protein machinery used by various pathogenic Gram-negative bacteria to transport virulence factors into the host cell during infection. Understanding the structure of T3SSs is crucial for future developments of therapeutics that could target this system. However, much of the knowledge about the structure of T3SS is available only for Salmonella, and it is unclear how this large assembly is conserved across species. Here, we combined cryo-electron microscopy, cross-linking mass spectrometry, and integrative modeling to determine the structure of the T3SS needle complex from Shigella flexneri. We show that the Shigella T3SS exhibits unique features distinguishing it from other structurally characterized T3SSs. The secretin pore complex adopts a new fold of its C-terminal S domain and the pilotin MxiM[SctG] locates around the outer surface of the pore. The export apparatus structure exhibits a conserved pseudo-helical arrangement but includes the N-terminal domain of the SpaS[SctU] subunit, which was not present in any of the previously published virulence-related T3SS structures. Similar to other T3SSs, however, the apparatus is anchored within the needle complex by a network of flexible linkers that either adjust conformation to connect to equivalent patches on the secretin oligomer or bind distinct surface patches at the same height of the export apparatus. The conserved and unique features delineated by our analysis highlight the necessity to analyze T3SS in a species-specific manner, in order to fully understand the underlying molecular mechanisms of these systems. This article is protected by copyright. All rights reserved. | ||
| - | + | Integrative structural analysis of the type III secretion system needle complex from Shigella flexneri.,Flacht L, Lunelli M, Kaszuba K, Chen ZA, O' Reilly FJ, Rappsilber J, Kosinski J, Kolbe M Protein Sci. 2023 Feb 15:e4595. doi: 10.1002/pro.4595. PMID:36790757<ref>PMID:36790757</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Lunelli | + | <div class="pdbe-citations 8axn" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Shigella flexneri]] | ||
| + | [[Category: Lunelli M]] | ||
Current revision
Inner membrane ring and secretin N0 N1 domains of the type 3 secretion system of Shigella flexneri
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