7z1l
From Proteopedia
(Difference between revisions)
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<StructureSection load='7z1l' size='340' side='right'caption='[[7z1l]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='7z1l' size='340' side='right'caption='[[7z1l]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[7z1l]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_W303 Saccharomyces cerevisiae W303]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z1L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7z1l]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_W303 Saccharomyces cerevisiae W303] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z1L FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4QM:(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-DIMETHYL-17-[(2R)-PENTAN-2-YL]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-TETRADECAHYDRO-1H-CYCLOPENTA[A]PHENANTHRENE-3,7,12-TRIOL'>4QM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4QM:(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-DIMETHYL-17-[(2R)-PENTAN-2-YL]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-TETRADECAHYDRO-1H-CYCLOPENTA[A]PHENANTHRENE-3,7,12-TRIOL'>4QM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z1l OCA], [https://pdbe.org/7z1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z1l RCSB], [https://www.ebi.ac.uk/pdbsum/7z1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z1l ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z1l OCA], [https://pdbe.org/7z1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z1l RCSB], [https://www.ebi.ac.uk/pdbsum/7z1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z1l ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/RPC10_YEAST RPC10_YEAST] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Involved in Pol III transcription reinitiation and RNA cleavage during transcription termination. | |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RNA polymerase (Pol) III is specialized to transcribe short, abundant RNAs, for which it terminates transcription on polythymine (dT) stretches on the non-template (NT) strand. When Pol III reaches the termination signal, it pauses and forms the pre-termination complex (PTC). Here, we report cryoelectron microscopy (cryo-EM) structures of the yeast Pol III PTC and complementary functional states at resolutions of 2.7-3.9 A. Pol III recognizes the poly(dT) termination signal with subunit C128 that forms a hydrogen-bond network with the NT strand and, thereby, induces pausing. Mutating key interacting residues interferes with transcription termination in vitro, impairs yeast growth, and causes global termination defects in vivo, confirming our structural results. Additional cryo-EM analysis reveals that C53-C37, a Pol III subcomplex and key termination factor, participates indirectly in Pol III termination. We propose a mechanistic model of Pol III transcription termination and rationalize why Pol III, unlike Pol I and Pol II, terminates on poly(dT) signals. | ||
| + | |||
| + | Architecture of the yeast Pol III pre-termination complex and pausing mechanism on poly(dT) termination signals.,Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW Cell Rep. 2022 Sep 6;40(10):111316. doi: 10.1016/j.celrep.2022.111316. PMID:36070694<ref>PMID:36070694</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7z1l" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae W303]] | [[Category: Saccharomyces cerevisiae W303]] | ||
| + | [[Category: Synthetic construct]] | ||
[[Category: Girbig M]] | [[Category: Girbig M]] | ||
[[Category: Mueller CW]] | [[Category: Mueller CW]] | ||
Current revision
Structure of yeast RNA Polymerase III Pre-Termination Complex (PTC)
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