1i94

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[[Image:1i94.gif|left|200px]]
 
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==CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3==
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The line below this paragraph, containing "STRUCTURE_1i94", creates the "Structure Box" on the page.
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<StructureSection load='1i94' size='340' side='right'caption='[[1i94]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1i94]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I94 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=WO2:OCTADECATUNGSTENYL+DIPHOSPHATE'>WO2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1i94| PDB=1i94 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i94 OCA], [https://pdbe.org/1i94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i94 RCSB], [https://www.ebi.ac.uk/pdbsum/1i94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i94 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS12_THET8 RS12_THET8] With S4 and S5 plays an important role in translational accuracy (By similarity).[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.[HAMAP-Rule:MF_00403_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/1i94_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i94 ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3'''
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==See Also==
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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*[[Ribosome|Ribosome]]
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==Overview==
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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The small ribosomal subunit is responsible for the decoding of genetic information and plays a key role in the initiation of protein synthesis. We analyzed by X-ray crystallography the structures of three different complexes of the small ribosomal subunit of Thermus thermophilus with the A-site inhibitor tetracycline, the universal initiation inhibitor edeine and the C-terminal domain of the translation initiation factor IF3. The crystal structure analysis of the complex with tetracycline revealed the functionally important site responsible for the blockage of the A-site. Five additional tetracycline sites resolve most of the controversial biochemical data on the location of tetracycline. The interaction of edeine with the small subunit indicates its role in inhibiting initiation and shows its involvement with P-site tRNA. The location of the C-terminal domain of IF3, at the solvent side of the platform, sheds light on the formation of the initiation complex, and implies that the anti-association activity of IF3 is due to its influence on the conformational dynamics of the small ribosomal subunit.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1I94 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I94 OCA].
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==Reference==
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Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3., Pioletti M, Schlunzen F, Harms J, Zarivach R, Gluhmann M, Avila H, Bashan A, Bartels H, Auerbach T, Jacobi C, Hartsch T, Yonath A, Franceschi F, EMBO J. 2001 Apr 17;20(8):1829-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11296217 11296217]
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[[Category: Protein complex]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Avila, H.]]
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[[Category: Avila H]]
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[[Category: Bartels, H.]]
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[[Category: Bartels H]]
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[[Category: Franceschi, F.]]
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[[Category: Franceschi F]]
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[[Category: Gluehmann, M.]]
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[[Category: Gluehmann M]]
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[[Category: Harms, J.]]
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[[Category: Harms J]]
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[[Category: Hartsch, T.]]
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[[Category: Hartsch T]]
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[[Category: Jacobi, C.]]
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[[Category: Jacobi C]]
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[[Category: Pioletti, M.]]
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[[Category: Pioletti M]]
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[[Category: Schluenzen, F.]]
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[[Category: Schluenzen F]]
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[[Category: Yonath, A.]]
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[[Category: Yonath A]]
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[[Category: Zarivach, R.]]
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[[Category: Zarivach R]]
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[[Category: 30s ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:43:33 2008''
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Current revision

CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3

PDB ID 1i94

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