7uw9
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7uw9]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrus_limon Citrus limon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UW9 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7uw9]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrus_limon Citrus limon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UW9 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uw9 OCA], [https://pdbe.org/7uw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uw9 RCSB], [https://www.ebi.ac.uk/pdbsum/7uw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uw9 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uw9 OCA], [https://pdbe.org/7uw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uw9 RCSB], [https://www.ebi.ac.uk/pdbsum/7uw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uw9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == | + | <div style="background-color:#fffaf0;"> |
| - | + | == Publication Abstract from PubMed == | |
| + | We used the Legionella pneumophila effector SidK to affinity purify the endogenous vacuolar-type ATPases (V-ATPases) from lemon fruit. The preparation was sufficient for cryoelectron microscopy, allowing structure determination of the enzyme in two rotational states. The structure defines the ATP:H(+) ratio of the enzyme, demonstrating that it can establish a maximum DeltapH of approximately 3, which is insufficient to maintain the low pH observed in the vacuoles of juice sac cells in lemons and other citrus fruit. Compared with yeast and mammalian enzymes, the membrane region of the plant V-ATPase lacks subunit f and possesses an unusual configuration of transmembrane alpha helices. Subunit H, which inhibits ATP hydrolysis in the isolated catalytic region of V-ATPase, adopts two different conformations in the intact complex, hinting at a role in modulating activity in the intact enzyme. | ||
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| + | Structure of V-ATPase from citrus fruit.,Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL Structure. 2022 Oct 6;30(10):1403-1410.e4. doi: 10.1016/j.str.2022.07.006. Epub , 2022 Aug 29. PMID:36041457<ref>PMID:36041457</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7uw9" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[ATPase 3D structures|ATPase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Citrus V-ATPase State 1, H in contact with subunit a
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